Department Head and Distinguished Research Professor, Adjunct CCRC Member Member of the Center for Tropical and Emerging Global Diseases Adjunct Professor of Microbiology Education Education: B.S. with Honors in Chemistry, University of Florida, 1973 Ph.D., NSF Pre-doctoral Fellow in Cell Biology, Caltech, 1978 American Cancer Society Post-doctoral Fellow, University of Pennsylvania, 1977-79 Research Research Areas: Glycobiology Developmental Biology Medicinal Chemistry & Chemical Biology Structural Biology Labs: West Selected Publications Selected Publications: All publications:see: http://www.ncbi.nlm.nih.gov/sites/myncbi/1LOb8OnUO-7kI/bibliography/40443676/public/?sort=date&direction=ascending Recent publications (2014–): Xu, X., A. Eletsky, M.O. Sheikh, J.H. Prestegard & C.M. West (2018) Glycosylation promotes the random coil to helix transition in a region of a protist Skp1 associated with F-box binding. Biochemistry 57:511-515 Sheikh, M.O., D. Thieker, G. Chalmers, C.M. Schafer, M. Ishihara, P. Azad2, R.J. Woods, J.N. Glushka, B. Bendiak, J.H. Prestegard & C.M. West (2017) O2 sensing–associated glycosylation exposes the F-box–combining site of the Dictyostelium Skp1 subunit in E3 ubiquitin ligases. J. Biol. Chem. 292:18897-18915. PMCID: PMC5704474 West, C.M. & G.W. Hart (2017) Nucleocytoplasmic Glycosylation. In: Varki, A., Cummings, R.D., Esko, J.D., Stanley, P., Hart, G.W., Aebi, M., Darvill, A.G., Kinoshita, T., Packer, N.H., Prestegard, J.H., Schnaar, R.L., Seeberger, P.H., editors. Essentials of Glycobiology [Internet]. 3rd edition. Cold Spring Harbor Laboratory Press; Chapter 18. West, C.M. (2015) The 2015 Karl Meyer Lectureship Award and the Rosalind Kornfeld Award for Lifetime Achievement in Glycobiology, from the Society for Glycobiology. Glycobiology 25:1137-1138. Rahman, K., M. Mandalasi, P. Zhao, M.O. Sheikh, R. Taujale, H.W. Kim, H. van der Wel, K. Matta, N. Kannan, J.N. Glushka, L. Wells & C.M. West (2017) Characterization of a cytoplasmic glucosyltransferase that extends the core trisaccharide of the Toxoplasma Skp1 E3 ubiquitin ligase subunit. J. Biol. Chem. 292:18644-18659. PMCID: PMC5682971 Taylor-Mulneix, D.L., L. Bendor, B. Linz, I. Rivera, V.E. Ryman, K.K. Dewan, S.M. Wagner, E.F. Wilson, L.J. Hilburger, L.E. Cuff, C.M. West & E.T. Harvill (2017) Bordetella bronchiseptica exploits the complex life cycle of Dictyostelium discoideum as an amplifying transmission vector. PLoS Biol. 15:e2000420. PMCID: PMC5389573 Sheikh, M.O., S.M. Halmo, S. Patel, D. Middleton, H. Takeuchi, C.M. Schafer, C.M. West, R.S. Haltiwanger, F.Y. Avci, K.W. Moremen & L. Wells (2016) Rapid screening of sugar-nucleotide donor specificities of putative glycosyltransferases. Glycobiology 27:206-212 Summers, J.A., A.R. Harper, C.L. Feasley, H. van der Wel, J.N. Byrum, M. Hermann & C.M. West (2016) Identification of apolipoprotein A-1 as a retinoic acid binding protein in the eye. J. Biol. Chem. 291:18991-19005. PMCID: PMC5009271 Bergstrom, K., X. Liu, Y. Zhao, N. Gao, Q. Wu, K. Song, Y. Cui, Y. Li, J.M. McDaniel, S. McGee, W. Chen, M.M. Huycke, C.W. Houchen, L.A. Zenewicz, C.M. West, H. Chen, J. Braun, J. Fu & L. Xia (2016) Defective intestinal mucin-type O-glycosylation causes spontaneous colitis-associated cancer in mice. Gastroenterology 151:152-164. PMCID: PMC5068133 Rahman K., P. Zhao, M. Mandalasi, H. van der Wel, L. Wells, I.J. Blader & C.M. West (2016) The E3-ubiquitin ligase adaptor protein Skp1 is glycosylated by an evolutionarily conserved pathway that regulates protist growth and development. J. Biol. Chem. 291:4268-4280. PMCID: PMC4813455 *Designated ‘Paper of the Week’ West, C.M. & I.J. Blader (2015) Oxygen sensing by protozoans: How they catch their breath. Curr. Opin. Microbiol. 26:41-47 Sheikh, M.O., Y Xu, H. van der Wel, P. Walden, S.D. Hartson & C.M. West (2015) Glycosylation of Skp1 promotes formation of Skp1/Cullin-1/F-box protein complexes in Dictyostelium. Mol. Cell. Proteomics 14:66-80. PMC4288264 Weigel, P.H., C.M. West, P. Zhao, L. Wells, B.A. Baggenstoss & J.L. Washburn (2015) Hyaluronan synthase assembles chitin oligomers with –GlcNAc(α1–)UDP at the reducing end. Glycobiology 25:632-643 (PMC4410831) Feasley, C.L., H. van der Wel & C.M. West (2015) Evolutionary diversity of social amoebae N-glycomes may support interspecific autonomy. Glycoconjugate J. 32:345–359. Chinoy, Z.S., C.M. Schafer, C.M. West & G.-J. Boons (2015) Chemical synthesis of a glycopeptide derived from Skp1 for probing protein specific glycosylation. Chemistry – A European Journal 21:11779-11787 (PMC4646072) Koeller, C.M., H. van der Wel, C.L. Feasley, F. Abreu, F. Montalvão, P. Fampa, F.C.G. dos Reis, G.C. Atella, T. Souto-Padrón, C.M. West* & N. Heise* (2014) Non-enzymatic function of UDP-GlcNAc:polypeptide O-α-N-acetyl-D-glucosaminyltransferase-2 (TcOGNT2) regulates metacyclogenesis in Trypanosoma cruzi. Eukaryotic Cell 13:1312-1327. *co-corresponding authors (PMC4187647) Karunaratne, C.V., T. Weldeghiorghis, C.M. West & C.M. Taylor (2014) Conformational changes associated with post-translational modifications of Pro143 in Skp1 of Dictyostelium- a dipeptide model system. J. Am. Chem. Soc. 136:15170-15175 (PMC4227711) West, M.B., K. Partyka, C.L. Feasley, K.A. Maupin, I. Goppallawa, C.M. West, B.B. Haab & M.H. Hanigan (2014) Detection of distinct glycosylation patterns on human γ-glutamyl transpeptidase 1 using antibody-lectin sandwich array (ALSA) technology. BMC Biotechnology 14:101 (PMC4297448) Pan, Y., T. Yago, J. Fu, B. Herzog, J.M. McDaniel, P. Mehta-D’Souza, C. Ruan, R.P. McEver, C.M. West, K. Dai, H. Chen & L. Xia (2014) Podoplanin requires sialylated O-glycan for stable expression on lymphatic endothelial cells and for interaction with platelets. Blood 124:3656-3665 (PMC4256915) Schafer, C.M., M.O. Sheikh, D. Zhang & C.M. West (2014) Novel regulation of Skp1 by the Dictyostelium AgtA α-galactosyltransferase involves the Skp1-binding activity of its WD40-repeat domain. J. Biol. Chem. 289:9076-9088 (PMC3979368) Sheikh, M.O., C.M. Schafer, J.T. Powell, K.K. Rodgers, B.H.M. Mooers & C.M. West (2014) Glycosylation of Skp1 affects its conformation and promotes binding to a model F-box protein. Biochemistry 53:1657-1669. (PMC3985704) West, C.M., H. van der Wel, Z. Chinoy, G.-J. Boons, T.J. Gauthier, C.M. Taylor & Y. Xu (2014) Generating isoform-specific antibodies: lessons from the nucleocytoplasmic glycoprotein Skp1. In: Glycoscience: Biology and Medicine. Eds.: N. Taniguchi, T. Endo, G. Hart, P. Seeberger, C.-H. Wong. (Springer), pp. 927-934. Courses Regularly Taught Courses Regularly Taught: BCMB 4010 (Undergraduate) / BCMB 6010 (Graduate) BCMB 8060 Of Note Of note: Current outside activities: Editorial Board member: BBA General Subjects, Glycobiology Board Member: Association of Medical and Graduate Departments of Biochemistry NIH Study Section reviewer, ad hoc Grants Grants: Extramural funding (as PI): NIH 5 R01 GM-037539-25; Cytosolic proline hydroxylation and glycosylation (2013-2018) NIH 2R01 GM-084383-07 (with I. Blader); Glycoregulation of Skp1 in the cytoplasm and nucleus (2015-2019) NIH 1R21 AI-123161-01 (with R. Tarleton, L. Wells); Enabling tools for protist pathogen glycobiology (2015-2018) NIH 2R01AI069986-12 (subcontract to I.J. Blader); Control of Toxoplasma gondii Growth by the Host Cell Transcription Factor HIF1 (2017-2020) Recent past funding (as PI): NIH 1R21 AI-109643-01 (with I. Blader); Role of rolyl hydroxylation in Toxoplasma oxygen sensing (2015-2017) NIH 1R03TW008725-04 (Fogarty); Role of mucin-type O-glycosylation in Trypanosoma cruzi (2011-2014) NIH 1S06GM110786 (Indian Health Services); Oklahoma NARCH Student Development Program- NARCH VIII (2014-2015) Mizutani Foundation for Glycoscience 14-140; Role of cytoplasmic glycans in protozoan oxygen sensing (2015)
