CURRICULUM VITAE (updated January 2, 2014) J. David Puett, Ph.D. CURRENT APPOINTMENTS AND CONTACT INFORMATION Regents Professor and Department Head Emeritus Department of Biochemistry and Molecular Biology The University of Georgia Athens, Georgia 30602 Adjunct Professor Department of Biochemistry and Biophysics The University of North Carolina at Chapel Hill School of Medicine Chapel Hill, North Carolina 27599 Email <:puett@bmb.uga.edu>; <jdpuett@med.unc.edu> EDUCATION 1969 Ph.D. in Biochemistry, University of North Carolina at Chapel Hill, NC 1965 M.S. in Physics, University of North Carolina at Chapel Hill, NC 1961 B.S. in Physics, North Carolina State University, Raleigh, NC ACADEMIC APPOINTMENTS 2013-present Adjunct Professor of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 2010-present Regents Professor and Department Head Emeritus, Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA (August 1, 2010) 2001-2010 Regents Professor, Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 1993-2001 Professor, Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 1993-2006 Head, Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA 1993-1995 Adjunct Professor of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 1983–1993 Director, The Reproductive Sciences and Endocrinology Laboratories, University of Miami School of Medicine, Miami, FL (primary appointment) 1983-1993 Professor of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL (primary appointment) 1983-1993 Professor of Medicine and of Obstetrics and Gynecology, University of Miami School of Medicine, Miami, FL (secondary appointments) 1983-1986 Adjunct Professor of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 1979-1983 Professor of Biochemistry, Vanderbilt University School of Medicine, Nashville,TN 1974-1979 Associate Professor of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 1969-1974 Assistant Professor of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN OTHER RELEVANT PROFESSIONAL APPOINTMENTS AND EMPLOYMENT 2006 Member of the Athens Regional Medical Center Institutional Review Board 2005-2010 Member of the Center for Drug Discovery Executive Committee 2005-2006 President of the Association of Medical & Graduate Departments of Biochemistry 2005 Coordinator of the International Conference on Gonadotropins & Receptors, University of Georgia 2004-2005 President-Elect of the Association of Medical and Graduate Departments of Biochemistry 2004-2012 Member of the Athens Regional Medical Center Oncology Committee 2000-2003 Coordinator of the University of Georgia’s Cancer Initiative and Partnership with MCG and the Athens medical community. 2000-2002 Chair of the Education Committee and Member of the Board of Directors, Association of Medical and Graduate Departments of Biochemistry 2000-2001 Honors Program Faculty Mentor, University of Georgia 2000-2007 Advisory Board Member of the Center for Undergraduate Research Opportunities, University of Georgia 1999-2003 Senior Foundation Fellow, University of Georgia (Emeritus: 2003-2010) 1999 Visiting Professor at Benedict College, Columbia, SC, sponsored by the Endocrine Society 1995-1996 Advisory Board, Georgia Biotechnology Center, Georgia Research Alliance 1993-1996 Advisory Board, The Vanderbilt University Center for Reproductive Biology Research 1993 Bohan Visiting Professor, University of Kansas 1990 Consultant to the World Health Organization to Evaluate Institutes in Shanghai and Beijing 1990 Visiting Professor in the Glycoprotein Hormone Course, University of Michigan, Ann Arbor 1989-1991 National Scientific Advisory Board of the Women's Research Institute, University of Kansas, Wichita, KS 1988 Society of Tokyo Women's Medical College, Visiting Professor 1988 Visiting Professor, Zhejiang Medical University, China 1985-1993 Program Leader, The University of Miami Comprehensive Cancer Center 1985-1990 Scientific Advisory Board of the Cancer Research Council, Bethesda, MD 1984-1993 Program Director, The Reproductive Biology Training Program, University of Miami, FL 1984-1993 Member, Comprehensive Cancer Center, University of Miami, FL 1980-1983 Medical Advisory Board, National Hormone and Pituitary Program 1977-1979 Director of Graduate Studies, Department of Biochemistry, Vanderbilt University 1975-1983 Program Director, Vanderbilt Population Training Program 1975-1983 Member of the Vanderbilt Cancer Center 1975-1976 Vice-Chairman, Vanderbilt Graduate Faculty Council 1974-1976 Member, Exec. Comm. of the Vanderbilt Graduate Faculty Council 1973-1983 Associate Director and Member, Vanderbilt Population Center 1973-1976 Member, Vanderbilt Graduate Faculty Council 1962-1967 Research Associate, Basic Research Department, Chemstrand Research Center, Inc. (Monsanto subsidiary), Research Triangle Park, NC 1961-1962 Research Scientist, Bancroft Co., Wilmington, DE NATIONAL INSTITUTES OF HEALTH STUDY SECTION APPOINTMENTS 1991-1993 Chairperson, Biochemical Endocrinology Study Section (Member, 1990-1993) 1979-1982 Biochemical Endocrinology Study Section Member 1977-1979 Reproductive Biology Study Section (AHR) ad hoc Member Also Invited speaker to several workshops supported by NICHHD; invited to assist in writing RFP for NIH rat pituitary hormone program; invited to review applications for several NIH contracts; member of numerous NIH site visit teams and ad hoc Member of several Study Sections AMERICAN CANCER SOCIETY REVIEW PANEL APPOINTMENT 1994-1997 Tumor Biochemistry and Endocrinology Study Section ADDITIONAL GRANT REVIEW ACTIVITIES In addition to regular and ad hoc memberships on NIH Study Sections and the American Cancer Society Tumor Biochemistry and Endocrinology Review Panel, Dr. Puett regularly reviewed grants for the National Science Foundation, the U.S. Department of Agriculture, and funding agencies in Israel and Poland. EDITORIAL BOARD APPOINTMENTS 2011-present Editorial Advisory Board, Annual Review and Research in Biology 2010-present Editorial Board, Biomolecules (MDPI) 2003-2006 Editorial Board, Endocrine Reviews 2003-2008 Editorial Board, Molecular Endocrinology 2003-2006 Editorial Advisory Board, Current Trends in Endocrinology 1996-1999 Editorial Board, Endocrinology 1993-2010 Editorial Board, Endocrine 1983-1986 Editorial Board, Journal of Andrology 1982-1987 Editorial Board, Journal of Biological Chemistry Also reviewer of manuscripts for Biochemistry, Molecular Endocrinology, Endocrinology, Journal of Biological Chemistry, The FASEB Journal, International Journal of Peptide and Protein Research, Molecular and Cellular Endocrinology, Chemical Reviews, Archives of Biochemistry and Biophysics, Journal of Clinical Endocrinology and Metabolism, Proceedings of the National Academy of Sciences, Endocrine Reviews, Journal of Andrology, Science, Gene, Journal of Molecular Endocrinology, Biopolymers, and BioEssays. Consultant (ad hoc) to Oxford University Press and CRC Press, Inc. HONORS AND AWARDS 2007 Joy Goodwin Lecturer, Auburn University 2005 Honorary Professor, Jilin University, Changchun, China 2005 Neena B. Schwartz Lectureship in Reproductive Science, Northwestern University 2004 Catalyst Award, The Georgia Research Alliance 2001 Regents Professor, University of Georgia 1992 John M. Marsh Memorial Lecturer, University of Miami 1992 Omicron Delta Kappa (National Leadership Honor Society), University of Miami 1991 Phi Beta Delta (Honor Society for International Scholars), University of Miami 1990 Robert B. Greenblatt Lecturer, Medical College of Georgia, Augusta 1986 George Paff Award for Teaching Excellence, University of Miami School of Medicine 1984 Commissioned by the United Nations Fund for Population Activities to edit a book entitled "Human Fertility, Health and Food. Impact of Molecular Biology and Biotechnology" for the 1984 International Conference on Population, Mexico City 1975 Research Career Development Awardee (NIH) 1971 Camille and Henry Dreyfus Teacher-Scholar Awardee (Dreyfus Foundation) PROFESSIONAL AND LEARNED SOCIETIES (Member: Past aand Present) American Association for the Advancement of Science, American Association for Cancer Research, American Chemical Society, American Society for Biochemistry & Molecular Biology, Endocrine Society, Protein Society, Society for Ethnobiology, Society for the Study of Reproduction, The Renaissance Society TEACHING RESPONSIBILITIES At the University of Georgia Dr. Puett participated in several undergraduate and graduate courses, including service as course organizer and lead instructor for human biochemistry and disease, biology for medicine, and Florence and the Italian Renaissance. He also lectured first-year medical students at the Georgia Regents University/University of Georgia School of Medicine. At the University of Miami he participated in undergraduate, graduate, and medical student teaching. Topics in physical biochemistry, metabolism, and endocrinology were presented to undergraduate and graduate students; an integrated endocrinology course was developed and coordinated for first-year medical students. At Vanderbilt University he taught a course for graduate students in physical chemistry of biopolymers and presented lectures in endocrinology and neuroendocrinology to first-year medical and graduate students; he also taught in the biochemistry laboratory sections. Dr. Puett directed the Ph.D. dissertations of 26 students and the M.S. theses of seven students, has mentored nearly 30 postdoctoral fellows and visiting scientists, and has served on the Continuing Committees of numerous Ph.D. students in various departments at Vanderbilt University, the University of Miami, and the University of Georgia. Courses taught during the past several years: BCMB 4120/6120, Human Biochemistry and Disease, Fall (enrolled over 130 students in most recent offering) BCMB/GENE, Biology for Medicine, each Maymester (with Dr. Anderson; enrolled 30-35 students) HONS1990H, Renaissance Florence (Honors seminar (capped at 15 students) Medical School (first-year) lectures on endocrinology (40 students) FRES 1010, Florence, Epicenter of the Italian Renaissance (capped at 15 students) FRES 1010, The Language of Reproduction: Hormones as Messengers (capped at 15 students) FRES 1010, The Language of Human Reproductive Endocrinology (capped at 15 students) Guest lecturer in BCMB 4200/6200, Biotechnology; BIOL 2990H, Honors Seminar; GENE 3200H section, GENE Senior Seminar, and Endocrinology section for first-year medical students (GRU/UGA). OTHER ACTIVITIES Testified before the House Committee on Appropriations, Subcommittee, Departments of Labor and HEW, supporting appropriation for the Center for Population Research, NICHHD (4/25/79). Program Chairman of the 1986 Miami Winter Symposium on Advances in Gene Technology: Molecular Biology of the Endocrine System, Miami, FL (1/86). Co-organizer of a satellite symposium of the American Society of Cell Biology Meeting on Hormones, Cell Biology and Cancer, Washington, D.C. (12/86). Organizing Committee of the 1987 International Aromatase Conference, Miami, FL (3/87). Editor of the newsletter, The REPSCEND Reporter. Organizer of the W.J. LeMaire Symposium on Reproductive Endocrinology, Miami, FL (12/88) and Editor of the Proceedings (Steroids 54, November and December issues, 1989). Appearances on local (Miami channels 6 and 10) and national television (CNN). Co-Organizer of the Serono Symposium on Glycoprotein Hormones: Structure, Function and Clinical Implications, Santa Barbara, CA (3/93). Program Committee Member of the Society for the Study of Reproduction Annual Meeting (2002- 2004). RECENT FUNDING (J.D. Puett, PI) 1. NIH RO1 DK33973, hCG LH/CG Receptor Binding and Activation,” (approved through 4/30/10). [N.B. This grant was initially awarded to Dr. Puett at Vanderbilt University in 1972; it was then transferred to the University of Miami in 1983 and then to the University of Georgia in 1993.] Supplement awarded to DK33973 for research on human embryonic stem cells (7/10/03 - 4/30/04), direct costs: $75,000. Terminal fiscal year direct costs: $329,820. 2. NIH RO1 DK69711, “Binding Determinants of Glycoprotein Hormone Receptors,” 2005-2008 (no-cost extension approved through 12/31/11). Terminal fiscal year direct costs: $293,882. 3. Earlier funding included NIH R01s from NCI and GMS, two T32 Training grants, the Camille and Henry Dreyfus Foundation, and private funding from Smith-Kline. PATENTS 1. D. Puett, L.S. Kelly, and J.M. Pierce, “Lectin-Based Immunoassay for the Detection of hCG-Related Cancers,” U.S. Provisional Patent Application No. 60/370, 589 (2008). 2. J. Cui, F. Li, D. Puett, and Y. Xu, ``Protein Markers Identification for Gastric Cancer Diagnosis,`` U.S. Provisional Patent Application (2010). 3. J. Cui, F. Li, D. Puett, and Y. Xu, ``Methods and Systems for Predicting Proteins that can be Secreted into Bodily Fluids`` (2009). TRAINING RECORD Ph.D. Students and Current Position (if known) Dissertation Title (Year) Mario Ascoli, Ph.D. Metabolic Fate of Luteinizing Hormone (1975) Professor Dept. Pharmacology Univ. of Iowa Iowa City, IA Lenore Kelly, Ph.D. Purification and Characterization of Agilent Technologies Pituitary-derived Growth Factors (1978) San Francisco, CA R. Glenn Hammonds, Jr., Ph.D. Structure and Function of Beta-Endorphin (1979) Research Scientist, Tularik South San Francisco, CA Deborah L. Segaloff, Ph.D. Characterization of the Desensitized State of Professor Perifused Leydig Tumor Cells (1980) Dept. of Physiology & Biophys. Univ. of Iowa Iowa City, IA Thomas W. Strickland, Ph.D. Contribution of Subunits to Glycoprotein Research Scientist Hormone Function: A Study Utilizing AMGEN, Inc. Hybrids (1981) Thousand Oaks, CA James L. Jones, M.D., Ph.D. Synthesis of Phenothiazines with Specific Univ. of Florida Reporter Groups for Magnetic Resonance Jacksonville, FL Spectroscopy with Applications to Calmodulin and Serum Albumin (1981) Paula B. Hewgley, Ph.D. Characterization of Spin Labeled Retired (Nashville, TN) Calmodulin as a Probe for Calcium Binding (1983) Susan Bane Hastie, Ph.D. The Mechanism of Colchicine and Analog Professor Binding to Tubulin: Spectral, Thermodynamic Dept. of Chemistry Equilibrium and Kinetic Properties (1983) SUNY-Binghamton Binghamton, NY David P. Giedroc, Ph.D. The Interaction of Calmodulin with β-Endorphin Professor and Chair and Derived Peptides (1984) Dept. of Chemistry Indiana University Bloomington, IN Arthur E. Jackson, Ph.D. Specific Spin Labeling of Calmodulin and Law Office of AEJ Characterization of the Products (1985) Stockton, NJ Edith S. Lovegren, M.D., Ph.D. β-Endorphin and α-N-Acetyl β-Endorphin: Internal Medicine Practice Synthesis, Conformation and Binding Parameters Denver, CO (1986) Mark E. Brandt, Ph.D. Aromatase and Rat Ovarian Steroidogenesis (1988) Associate Prof. of Chemistry Rose-Hulman Inst. Of Technology Terre Haute, IN Samer El-Deiry, M.D., Ph.D. Characterization of a Carboxy-terminal Endocrinology Practice Deletion Mutant of the Human Albany, New York Choriogonadotropin Beta Subunit (1989) Frank S. Czerwiec, M.D., Ph.D. Hormonal Influences on Gene Expression: Pharmaceutical firm Gonadotropin and Growth Factor Bethesda, MD Control of Oncogenes (1990) Fang Chen, Ph.D. Structure-function Relationships Research Scientist of Human Choriogonadotropin (1991) Univ. of Pennsylvania Philadelphia, PA Haiying Xia, Ph.D. Structure-function Relationships of Human CEO (start-up) Chorionic Gonadotropin Using Site-directed South San Francisco, CA Mutagenesis: Contact Sites for Holoprotein Formation and Receptor Binding (1994) Jianing Huang, Ph.D. Molecular Basis of the Human Research Scientist Choriogonadotropin-Receptor Rigel, Inc. Interaction (1995) South San Francisco, CA Adviye Ergul, M.D., Ph.D. Molecular Aspects of Endothelin-1 (1995) Professor Dept. of Physiology Georgia Health Sci. Univ. Augusta, GA Lizette M. Fernandez, Ph.D. Structure-Function Relationships of the Law Firm (intellectual property) Luteinizing Hormone/Chorionic Gonadotropin Atlanta, GA Receptor (1995) Chengbin Wu, Ph.D. Structural and Functional Studies of Human CSO, Head of R&D Chorionic Gonadotropin and its Receptor Shanghai CP Guojian through Protein Engineering (1997) Shanghai, China Carlos A. Alvarez, Ph.D. Structure-Function Analysis of a Conserved Pensacola Christian College Region in the Extracellular Domain of the Rat Pensacola, FL Luteinizing Hormone/Chorionic Gonadotropin (LH/CG) Receptor (1997) Neil Bhowmick, Ph.D. Structure-Function Analysis of the Binding and Associate Professor Ligand-Mediated Down-Regulation of the Dept. Medicine Lutropin and Endothelin-A Receptors (1998) Cedars-Sinai Med. Ctr., CA Gregory B. Fralish, Ph.D. Expression and Structural Characterization of Program Director the Extracellular Binding Domain of the G-Protein Clin. Translational Med. Res. Coupled, Luteinizing Hormone Receptor in University of Virginia Complex with Its Ligand Choriogonadotropin Charlottsville, VA (2002) Rebecca L. Schubert, Ph.D. Fusion Proteins Engineered to Characterize Science Teacher Glycoprotein Hormone-Receptor Interactions (MD) (2002) Thomas P. Meehan, Ph.D. In vivo Consequences of the Expression of Physicians Assistant Constitutively Active Luteinizing Hormone Indianapolis, IN Receptors (2003) Co-mentor with Dr. Prema Narayan Yongsheng Li Structural Aspects of the Lutropin Receptor (deceased 03/26/2007) Geneva DeMars GPCR-G Protein Coupling (2010) Instructor Armstrong Atlantic State Univ. Savannah, GA M.S. Students Thesis Title (Year) and Current Position (if known) James D. Luketich, M.S.,M.D. Effects of Human Choriogonadotropin on Department of Surgery Mitochondrial and Microsomal Cytochrome University of Pittsburgh, PA P-450 Levels in Mouse Testes (1983) Mark E. Brandt, M.S., Ph.D. Aromatase and Testosterone Metabolism in Assoc. Prof. Chemistry Selected Steroidogenic Tissues and the Rose-Hulman Inst. Tech. Effects of an Aromatase Inhibitor (1986) Terre Hute, IN Enrique J. Mendoza, M.S., M.D. Effects of an Aromatase Inhibitor on Rat Dept. of Medicine Uterine Estrogen Receptors (1987) (Endocrinology) Panama City, Panama Xiaodong Wu, M.S., Ph.D. Cloning a Designed, Novel Calmodulin Gene Unknown (1989) Hakan Bermek, M.S., Ph.D. Internalization and Degradation of [125I] Professor Endothelin-1 by Vascular Smooth Muscle A-10 Istanbul, Turkey Cells (1995) Leyi Shen, M.S. Structure-Function Relationships of Human Choriogonadotropin β-Subunit Using Site- Directed Mutagenesis: On the Role of Conserved Arg-68 in Hormone Secretion (1995) Margaret Shirley, M.S., M.D. Characterization of Trophoblast-like Cells Derived University of California, SF from Human Embryonic Stem Cells (2005) San Francesco, CA Hope Blackstone (M.S.) Differentiation of Human Embryonic Stem Cells Co-mentor with Dr. Stephen Dalton Fred Hutchinson Seattle, WA Postdoctoral Fellows Years Current Position Leslie A. Holladay, Ph.D. 1972-75 Retired (TN) Mario Ascoli, Ph.D. 1977-78 Professor Dept. Pharmacology Univ. of Iowa Iowa City, IA Thomas D. Noland, Ph.D. 1979-81 Unknown Michael H. Melner, Ph.D. 1980-83 Program Director American Cancer Society Atlanta, GA Stephen J. Zimniski, Ph.D. 1981-84 Associate Professor Dept. of Biology West Chester Univ. West Chester, PA Razia H. Ahmed, Ph.D. 1983-84 Unknown Arthur E. Jackson, Ph.D. 1985-86 Law Office of AEJ Stockton, NJ Henry G. Zot, Ph.D. 1986-88 Professor Dept. Physiology UT Southwestern Dallas, TX Mary H. Majercik, Ph.D. 1987-90 Deceased Satya P. Yadav, Ph.D. 1990-91 Unknown Fang Chen, Ph.D. 1990 Research Scientist Univ. of Pennsylvania Philadelphia, PA Marilyn K. Glassberg, M.D. 1991-92 Associate Professor Pulmonary Division Dept. of Medicine Univ. of Miami Miami, FL Kou-Cheng Peng, Ph.D. 1993-95 Senior Scientist Taipei, Taiwan Krassimira Angelova, Ph.D. 1993-94 Assoc. Research Scientist (retired) Dept. Biochemistry & Mol. Biol. Univ. of Georgia Athens, GA Adviye Ergul, M.D., Ph.D. 1995-1996 Professor Dept. Physiology Medical Coll. of GA Augusta, GA Lisa Kelly, Ph.D., D.V.M. 2000-2003 Veterinary Medicine Practice Athens, GA Vis. Sci. & Fac. Assoc. Years Current Position Ronald Brown, M.D. 1979 Professor of Medicine University of Oklahoma Oklahoma City, OK (Sabbatical) Zi-yi Liu, M.D. 1986-88 Professor & Head (retired) Dept. of Biochemistry Zhejiang Medical University Hangzhou, China (WHO Visiting Scientist) Shirley El-Maasarany, M.D.,Ph.D. 1988 Professor Dept. of Anatomy Alexandria University Alexandria, Egypt (Visiting Scientist) Yi Wang, B.S. 1989-90 Unknown Mary H. Majercik, Ph.D. 1990-91 Deceased Makoto Ujihara, M.D.,Ph.D. 1991-92 Dept. Medicine Tokyo Women's Medical College Tokyo, Japan Hiroaki Yoshida, M.D., Ph.D. 1991-93 Dept. of Ob/Gyn Sendai Univ. Sendai, Japan Marilyn K. Glassberg, M.D. 1992-93 Associate Professor Pulmonary Division Dept. of Medicine Univ. of Miami Miami, FL Prema Narayan, Ph.D. 1993-2005 Associate Professor Dept. of Physiology Southern Illinois University School of Medicine Carbondale, IL Do-Young Yoon, Ph.D. 1994 Scientist Genetic Engineering Research Inst. Taejon, Korea Krassimira Angelova, Ph.D. 1994-pres. Assoc. Res. Scientist Dept. Biochemistry & Mol. Biol. Univ. of Georgia Athens, GA Adviye Ergul, M.D., Ph.D. 1996-1999 Professor Dept. Physiology Medical College of Georgia Augusta, GA Susanne W. Warrenfeltz, Ph.D. 2004-2008 Science writer Athens, GA Axel P.N. Themmen, Ph.D. 2007 Associate Professor Department of Internal Medicine Eramus School of Medicine Rotterdam, The Netherlands (Sabbatical) BIBLIOGRAPHY Contents Page(s) Juried Research Publications 14-33 Juried Publication in Medical Education 33 Book Chapters, Reviews, and Symposium Proceedings 33-36 Short Reports 36-38 Essay 39 Books, Journals (Special Issues) Edited 39 Note on Published Abstracts and Meeting Presentations 39 JURIED PUBLICATIONS 1. D. Puett, K. Smith, A. Ciferri, and E. Kontos (1964) “Elasticity of Block Copolymers,” J. Chem. Physics 40, 253-254. 2. D. Puett, L. Smith and A. Ciferri (1965) “Elasticity of Semicrystalline Polymers,” J. Phys. Chem. 69, 141-150. 3. L. Rajagh, D. Puett, and A. Ciferri (1965) “Interaction Between Proteins and Salt Solutions. I. Thermodynamic Parameters of Dilution for Gelatin and Collagen,” Biopolymers 3, 421-437. 4. D. Puett, A. Ciferri, and L. Rajagh (1965) “Interaction Between Proteins and Salt Solutions. II. Elasticity of Collagen Tendons,” Biopolymers 3, 439-459. 5. A. Ciferri, L. Rajagh, and D. Puett (1965) “Interaction Between Proteins and Salt Solutions. III. Effect of Salt Type and Concentration on the Shrinkage Temperature,” Biopolymers 3, 461-480. 6. K. Smith and D. Puett (1966) “Stress-Optical Behavior of Rubber Networks at Large Deformations,” J. Appl. Physics 37, 346-354. 7. D. Puett, R. Garmon, and A. Ciferri (1966) “Role of Neutral Salts on the Denaturation Temperature of Biopolymers,” Nature 211, 1294-1295. 8. D. Puett (1967) “Thermoelasticity and the Temperature Coefficient of Unperturbed Polymer Dimensions,” Makromoleculare Chemie 100, 200-207. 9. D. Puett (1967) “DTA and Heats of Hydration of Some Polypeptides,” Biopolymers 5, 327-330. 10. E. Bianchi, G. Conio, A. Ciferri, D. Puett, and L. Rajagh (1967) “The Role of pH, Temperature, Salt Type, and Salt Concentration on the Stability of the Crystalline, Helical and Randomly Coiled forms of Collagen,” J. Biol. Chem. 242, 1361-1369. 11. A. Ciferri, R. Garmon, and D. Puett (1967) “Interaction Between Proteins and Salt Solutions. IV. Enrichment of Salts in Collagen During Denaturation,” Biopolymers 5, 439-456. 12. D. Puett (1967) "Network Behavior of Polyurethane Elastomers," J. Polymer Sci. (A-2) 5, 839-852. 13. D. Puett, A. Ciferri, E. Bianchi, and J. Hermans, Jr. (1967) "Helix Formation of Poly-L-Lysine Thiocyanate in Aqueous Solutions," J. Phys. Chem. 71, 4126-4128. 14. D. Puett and L. Rajagh (1968) "The Effect of Aqueous Salt Solutions on the Melting of Collagen and the Viscosity of Gelatin," Macromolecular Science (Chemistry) A-2, 111-133. 15. J. Threlkeld, J. Burke, D. Puett, and A. Ciferri (1968) "Determination of the Binding of Tetraalkyl Ammonium Bromides to Gelatin by an Ultracentrifuge Method," Biopolymers 6, 767-770. 16. A. Ciferri, D. Puett, L. Rajagh, and J. Hermans, Jr. (1968) "Potentiometric Titrations and the Helix-Coil Transition of Poly-L-Glumatic Acid and Poly-L-Lysine in Aqueous Salt Solutions," Biopolymers 6, 1019-1036. 17. D. Puett and A. Ciferri (1968) "Pseudo-Phase Diagrams of Poly-L-Lysine and Poly-L-Glutamic Acid in Aqueous Salt Solutions," Biopolymers 6, 1213-1217. 18. J. Hermans, Jr., D. Puett, and G. Acampora (1969) "On the Conformation of Denatured Proteins," Biochemistry 8, 22-30. 19. D. Puett and A. Ciferri (1971) "Conformational Studies on Concentrated Solutions of Poly-γ-Benzyl-L-Glutamate," Biopolymers 10, 547-564. 20. J. Hermans, Jr. and D. Puett (1971) "Relative Effects of Primary and Tertiary Structure on Helix Formation in Myoglobin and α-Lactalbumin," Biopolymers 10, 895-914. 21. W.L. Zahler, D. Puett, and S. Fleischer (1972) "Circular Dichroism of Mitochondrial Membranes Before and After Extraction of Lipids and Surface Proteins," Biochim. Biophys. Acta 255, 365-379. 22. T. Inagami, K. Murakami, D. Puett, A.S. Hartree, and A. Nureddin (1972) "N-Terminal Amino Acid Sequence of the α-Subunit of Human Pituitary Luteinizing Hormone," Biochem. J. 126, 441-442. 23. D. Puett (1972) "On the Formation of Secondary and Tertiary Structure in Apomyoglobin," Biochim. Biophys. Acta 257, 537-542. 24. D. Puett (1972) "A Study on the Conformation and Conformational Stability of Ribonuclease A and Its Peptic Derivative, Des-(121-124)-Ribonuclease," Biochemistry 11, 1980-1990. 25. D. Puett (1972) "Chain-Folding in Ribonuclease A Derivatives Lacking Five and Six Carboxyl-Terminal Residues," Biochemistry 11, 4304-4307. 26. D. Puett (1973) "Conformational Studies on a Glycosylated Bovine Pancreatic Ribonuclease," J. Biol. Chem. 248, 3566-3572. 27. D. Puett (1973) "The Equilibrium Unfolding Parameters of Horse and Sperm Whale Myoglobin," J. Biol. Chem. 248, 4623-4634. 28. D. Puett, B.K. Wasserman, J.D. Ford, and L.W. Cunningham (1973) "Collagen-Mediated Platelet Aggregation," J. Clin. Invest. 52, 2495-2506. 29. J.P. Robinson and D. Puett (1973) "Morphological and Conformational Studies of Tamm-Horsfall Urinary Glycoprotein," Arch. Biochem. Biophys. 159, 615-621. 30. D. Puett, E. Friebele, and R.G. Hammonds, Jr. (1973) "A Comparison of the Conformational Stabilities of Homologous Hemoproteins," Biochim. Biophys. Acta 328, 261-277. 31. L.A. Holladay, R.G. Hammonds, Jr., and D. Puett (1974) "Growth Hormone Conformation and Conformational Equilibria," Biochemistry 13, 1653-1661. 32. W.M. Moore, L.A. Holladay, D. Puett, and R.N. Brady (1974) "On the Conformation of the Acetylcholine Receptor Protein from Torpeda nobiliana," FEBS Letters 45, 145-149. 33. J.P. Robinson, L.A. Holladay, J.B. Picklesimer, and D. Puett (1974) "Tetanus Toxin Conformation," Mol. Cell. Biochemistry 5, 147-151. 34. M. Ascoli and D. Puett (1974) "Tritium Labeling of Luteinizing Hormone by Reductive Methylation," Biochim. Biophys. Acta 371, 203-210. 35. L.A. Holladay, J.H. Levine, W.E. Nicholson, D.N. Orth, W.D. Salmon, Jr., and D. Puett (1975) "Evidence for Structural Dissociation of Two Biologic Actions of Growth Hormone," Biochim. Biophys. Acta 381, 47-60. 36. M. Ascoli, R.A. Liddle, and D. Puett (1975) "The Metabolism of Luteinizing Hormone. Plasma Clearance, Urinary Excretion and Tissue Uptake," Mol. Cell. Endocrinology 3, 21-36. 37. J.P. Robinson, J.B. Picklesimer, and D. Puett (1975) "Tetanus Toxin. The Effect of Chemical Modifications on Toxicity, Immunogenicity, and Conformation," J. Biol. Chem. 250, 7435-7442. 38. L.A. Holladay and D. Puett (1975) "Gonadotropin and Subunit Conformation," Arch. Biochem. Biophys. 171, 708-720. 39. L.A. Holladay and D. Puett (1976) "Circular Dichroism of Corticotropin, Fragment 1-24, and Model Compounds. An Assessment of the Contributions of the Peptide Chromophore and Aromatic Residues," Biopolymers 15, 43-59. 40. L.A. Holladay and D. Puett (1976) "Somatostatin Conformation: Evidence for a Stable Intramolecular Structure from Circular Dichroism, Diffusion, and Sedimentation Equilibrium," Proc. Natl. Acad. Sci. USA 73, 1199-1202. 41. D. Puett, A. Nureddin, and L.A. Holladay (1976) "Circular Dichroism of Human Pituitary Luteinizing Hormone and Its Glycopeptides," International J. Peptide Protein Res. 8, 183-191. 42. L.A. Holladay, C.R. Savage, S. Cohen, and D. Puett (1976) "Conformation and Unfolding Thermodynamics of Epidermal Growth Factor and Derivatives," Biochemistry 15, 2624-2633. 43. M. Ascoli, R.A. Liddle, and D. Puett (1976) "Renal and Hepatic Lysosomal Catabolism of Luteinizing Hormone," Mol. Cell. Endocrinology 4, 297-310. 44. M. Ascoli and D. Puett (1976) "Biotransformations of Luteinizing Hormone in Serum and Urine. I. Association with Serum Components," Endocrinology 99, 1229-1236. 45. M. Ascoli and D. Puett (1976) "Biotransformations of Luteinizing Hormone in Serum and Urine. II. Evidence for Reduced Potency Following Urinary Excretion," Endocrinology 99, 1237-1243. 46. W.E. Nicholson, R.N. Barton, L.A. Holladay, D.N. Orth, and D. Puett (1977) "Tissue-Specific Stimulation of Ornithine Decarboxylase Activity by Pituitary Factors Immunologically Related to Growth Hormone," Endocrinology 100, 459-467. 47. D. Puett, L.A. Holladay, and J.P. Robinson (1977) "Circular Dichroism of Human Urinary Tamm-Horsfall Glycoprotein," Mol. Cell. Biochemistry 15, 109-116. 48. D. Puett, L.A. Holladay, J.D. Ford, and L.W. Cunningham (1977) "Circular Dichroism of Glycopeptide Fractions from α1-Acid Glycoprotein, Thyroglobulin, and Ovalbumin," Biochim. Biophys. Acta 491, 129-136. 49. D. Puett and L.A. Holladay (1977) "The Effects of Denaturant and Temperature on the Circular Dichroic Spectrum of Ovine Lutropin-α," International J. Peptide Protein Res. 10, 102-106. 50. J.P. Robinson, S. Derreberry, R.A. Liddle, M. Ascoli, and D. Puett (1977) "Renal Uptake of Lutropin," Mol. Cell. Biochemistry 15, 63-66. 51. M. Ascoli and D. Puett (1977) "Intracellular Uptake and Catabolism of Lutropin by Testicular Tissue in vivo," FEBS Letters 75, 77-82. 52. L.A. Holladay, M. Ascoli, and D. Puett (1977) "Conformational Stability and Self-Association of Zinc-Free Bovine Insulin at Neutral pH," Biochim. Biophys. Acta 494, 245-254. 53. L.A. Holladay and D. Puett (1977) "Comparative Conformational Analysis of Human Choriomammotropin and Somatotropin from Several Species," International J. Peptide Protein Res. 10, 363-368. 54. L.A. Holladay, J. Rivier, and D. Puett (1977) "Conformational Studies on Somatostatin and Analogs," Biochemistry 16, 4895-4900. 55. M. Ascoli and D. Puett (1978) "Gonadotropin Binding and Stimulation of Steroidogenesis in Leydig Tumor Cells," Proc. Natl. Acad. Sci. USA 75, 99-102. 56. N.V. Paniker, K.T.D. Lin, S.B. Krantz, J.M. Frexner, B.K. Wasserman, and D. Puett (1978) "Hemoglobin Vanderbilt (α2β289SerArg): A New Hemoglobin with High Oxygen Affinity and Compensatory Erythrocytosis," Br. J. Hematology 39, 249-258. 57. M. Ascoli and D. Puett (1978) "Degradation of Receptor-Bound Human Choriogonadotropin by Murine Leydig Tumor Cells," J. Biol. Chem. 253, 4892-4899. 58. D. Puett, J.H. Hash, and J.P. Robinson (1978) "Conformation of Diphtheria Toxin and an Enzymically-Active Fragment," FEBS Letters 89, 59-63. 59. W.E. Nicholson, R.A. Liddle, D. Puett, and G.W. Liddle (1978) "Adrenocorticotropic Hormone Biotransformation, Clearance, and Catabolism," Endocrinology 103, 1344-1351. 60. J.P. Robinson, H.C.J. Chen, J.H. Hash, and D. Puett (1978) "Enzymic Fragmentation of Tetanus Toxin. Identification and Characterization of an Atoxic, Immunogenic Fragment," Mol. Cell. Biochemistry 21, 23-31. 61. M. Ascoli and D. Puett (1978) "Inhibition of the Degradation of Receptor-Bound Human Choriogonadotropin by Lysosomotropic Agents, Protease Inhibitors and Metabolic Inhibitors," J. Biol. Chem. 253, 7832-7838. 62. J.O. McIntyre, L.A. Holladay, M. Smigel, D. Puett, and S. Fleischer (1978) "Hydrodynamic Properties of D-β-Hydroxybutyrate Dehydrogenase, a Lipid-Requiring Enzyme," Biochemistry 17, 4169-4177. 63. D. Puett, A. Kenner, R. Benveniste, and D. Rabinowitz (1978) "Characterization of the Human Chorionic Gonadotropin Fractions in Pregnancy Urine," Acta Endocrinol. 89, 612-624. 64. R. Benveniste, M.C. Conway, D. Puett, and D. Rabinowitz (1979) "Heterogeneity of the Human Chorionic Gonadotropin α-Subunit Secreted by Cultured Choriocarcinoma (JEG) Cells," J. Clin. Endocrinol. Metab. 48, 85-91. 65. D. Puett, R. Benveniste, A. Kenner, and D. Rabinowitz (1979) "The Carbohydrate Moiety of Human Chorionic Gonadotropin: Lack of Competition with HCG for Testicular Receptors and Anti-HCG Serum," Hormone Metabolic Res. 11, 165-167. 66. W.E. Nicholson, R.N. Barton, D. Puett, D.N. Orth, and G.W. Liddle (1979) "Evidence for Renotropic Activity in Ovine Pituitaries," Endocrinology 105, 16-20. 67. M.S. Runge, P.B. Hewgley, D. Puett, and R.C. Williams, Jr. (1979) "Cyclic Nucleotide Phosphodiesterase Activity in 10-nm Filaments and Microtubule Preparations from Bovine Brain," Proc. Natl. Acad. Sci. USA 76, 2561-2565. 68. A. LaCroix, M. Ascoli, D. Puett, and T.J. McKenna (1979) "Steroidogenesis in HCG-Responsive Leydig Cell Tumor Variants," J. Steroid Biochem. 10, 669-675. 69. R. Benveniste, J. Lindner, D. Puett, and D. Rabinowitz (1979) "Human Chorionic Gonadotropin α-Subunit from Cultured Choriocarcinoma (JEG) Cells: Comparison of the Subunit Secreted Free with that Prepared from Secreted HCG," Endocrinology 105, 581-587. 70. D.H. Albert, M. Ascoli, D. Puett, and J.G. Coniglio (1980) "Lipid Composition and Gonadotropin-Mediated Lipid Metabolism of the M5480 Murine Leydig Cell Tumor," J. Lipid Res. 21, 862-867. 71. L. Kelly and D. Puett (1980) "Preparation and Characterization of a Novel Group of Pituitary-Derived Peptides Stimulatory for DNA Synthesis in Fibroblasts," Biochim. Biophys. Acta 626, 397-411. 72. R.G. Hammonds, Jr., N. Ling, and D. Puett (1981) "Preparation of Tritium Labeled β-Endorphin Suitable for Studying Brain Receptor Interactions," Biochem. Biophys. Res. Commun. 98, 211-218. 73. D.L. Segaloff, D. Puett, and M. Ascoli (1981) "The Dynamics of the Steroidogenic Response of Perifused Leydig Tumor Cells to Human Chorionic Gonadotropin, Ovine Luteinizing Hormone, Cholera Toxin, and Adenosine 3',5'-Cyclic Monophosphate," Endocrinology 108, 632-638. 74. R.G. Hammonds, Jr., N. Ling, and D. Puett (1981) "Interaction of Tritiated β-Endorphin with Rat Brain Membranes," Anal. Biochem. 114, 75-84. 75. S. Gerard, D. Puett, and W.M. Mitchell (1981) "Kinetics of Collagen Fold Formation in Human Type I Procollagen and the Effect of Disulfide Bonds," Biochemistry 20, 1857-1865. 76. D.L. Segaloff, M. Ascoli, and D. Puett (1981) "Characterization of the Desensitized State of Leydig Tumor Cells," Biochim. Biophys. Acta 675, 351-358. 77. H.W. Detrich, III, R.C. Williams, Jr., T.L. Macdonald, L. Wilson, and D. Puett (1981) "Changes in the Circular Dichroic Spectrum of Colchicine Associated with its Binding to Tubulin," Biochemistry 20, 5999-6005. 78. T.W. Strickland and D. Puett (1981) "Contribution of Subunits to the Function of Luteinizing Hormone/Human Chorionic Gonadotropin Recombinants," Endocrinology 109, 1933-1942. 79. J.P. Robinson, L.A. Holladay, J.H. Hash, and D. Puett (1982) "Conformational and Molecular Weight Studies of Tetanus Toxin and Its Major Peptides," J. Biol. Chem. 256, 407-411. 80. T.D. Noland and D. Puett (1982) "Testicular Membranes with Improved Stability of the Gonadotropin Receptor," Biochim. Biophys. Acta 714, 226-233. 81. D. Puett, R.G. Hammonds, Jr., and N. Ling (1982) "Contribution of the Amino Terminal Tyrosine to the Interaction of β-Endorphin with Opiate Receptors," Peptides 3, 87-89. 82. D. Puett, R.J. Ryan, and V.C. Stevens (1982) "Circular Dichroic and Immunological Properties of Human Choriogonadotropin-β Carboxyl Terminal Peptides," International J. Peptide Protein Res. 19, 506-513. 83. T.W. Strickland and D. Puett (1982) "The Kinetic and Equilibrium Parameters of Subunit Association and Gonadotropin Dissociation," J. Biol. Chem. 257, 2954-2960. 84. R.G. Hammonds, Jr., A.S. Hammonds, N. Ling, and D. Puett (1982) "β-Endorphin and Deletion Peptides: A Correlation of Opiate Receptor Affinity with Helix Potential," J. Biol. Chem. 257, 2990-2995. 85. M.H. Melner, W.A. Lutin, and D. Puett (1982) "Epidermal Growth Factor and Cyclic AMP Stimulation of Distinct Protein Kinase Activities in Leydig Cell Tumor Membranes," Life Sci. 30, 1981-1986. 86. T.D. Noland, M.H. Melner, and D. Puett (1982) "Demonstration of Distinct Forms of Testicular Adenylate Cyclases Associated with Germinal and Leydig Cell Fractions," Biochim. Biophys. Acta 717, 183-189. 87. T.W. Strickland and D. Puett (1982) "α-Subunit Conformation in Glycoprotein Hormones and Recombinants as Assessed by Specific Antisera," Endocrinology 111, 95-100. 88. K. Nomura, D. Puett, W.E. Nicholson, and G.W. Liddle (1982) "Partial Purification and Characterization of a Renotropic Fraction from Ovine Pituitaries," Proc. Natl. Acad. Sci. USA 79, 6675-6679. 89. D.P. Giedroc, D. Puett, N. Ling, and J.V. Staros (1983) "Demonstration by Covalent Cross-Linking of a Specific Interaction between β-Endorphin and Calmodulin," J. Biol. Chem. 258, 16-19. 90. T.W. Strickland and D. Puett (1983) "Circular Dichroism of Gonadotropin Recombinants," International J. Peptide Protein Res. 21, 374-380. 91. J.D. Luketich, M.H. Melner, F.P. Guengerich, and D. Puett (1983) "Effects of Human Choriogonadotropin on Mitochondrial and Microsomal Cytochrome P-450 Levels in Mouse Testes," Biochem. Biophys. Res. Commun. 111, 424-429. 92. M.H. Melner, S.T. Sawyer, W.T. Evanochko, T.C. Ng, J.D. Glickson, and D. Puett (1983) "Phosphorus-31 Nuclear Magnetic Resonance Analysis of Epidermal Growth Factor Action in A-431 Human Epidermoid Carcinoma Cells and SV-40 Virus Transformed Mouse Fibroblasts," Biochemistry 22, 2039-2042. 93. M.H. Melner, S.J. Zimniski, and D. Puett (1983) "Divergent Effects of Phenothiazines on Leydig Tumor Cell Steroidogenesis and Adenylate Cyclase Activity," J. Steroid Biochem. 19, 1111-1117. 94. L. Dangott, D. Puett, and L.W. Cunninham (1983) "Conformational Changes Induced in Human α2-Macroglobulin by Protease and Nucleophilic Modification," Biochemistry 22, 3647-3653. 95. D. Puett, D.P. Giedroc, S. Tollefson, and N. Ling (1983) "des-(1-13) Human β-Endorphin Interacts with Calmodulin," Peptides 4, 191-194. 96. D.P. Giedroc, N. Ling, and D. Puett (1983) "Identification of β-Endorphin Residues 14-25 as a Region Involved in the Inhibition of Calmodulin-Stimulated Phosphodiesterase Activity," Biochemistry 22, 5584-5591. 97. L.J. Dangott, D. Puett, D.L. Garbers, and M.H. Melner (1983) "Tyrosine Protein Kinase Activity in Purified Rat Leydig Cells," Biochem. Biophys. Res. Commun. 116, 400-407. 98. S. Bane, D. Puett, T.L. MacDonald, and R.C. Williams, Jr. (1984) "Binding to Tubulin of the Colchicine Analog 2-Methoxy-5-(2',3',4',-trimethoxyphenyl)tropone. Thermodynamic and Kinetic Aspects," J. Biol. Chem. 259, 7391-7398. 99. M.H. Melner and D. Puett (1984) "Evidence for the Synthesis of Multiple Proopiomelanocortin-like Precursors in Murine Leydig Tumor Cells," Arch. Biochem. Biophys. 232, 197-201. 100. A.E. Jackson and D. Puett (1984) "Specific Acylation of Calmodulin. Synthesis and Adduct Formation with a Fluorenyl-based Spin Label," J. Biol. Chem. 259, 14985- 14992. 101. D.P. Giedroc, T.M. Keravis, J.V. Staros, N. Ling, J.N. Wells, and D. Puett (1985) "Functional Properties of Covalent β-Endorphin Peptide/Calmodulin Complexes. Chlorpromazine Binding and Phosphodiesterase Activation," Biochemistry 24, 1203- 1211. 102. M.H. Melner and D. Puett (1985) "Hormonal Induction of Specific Protein Synthesis in Murine Leydig Tumor Cells," Biochemistry International 10, 763-771. 103. S.J. Zimniski, M.H. Melner, and D. Puett (1985) "Induction of an Estrogen-dependent Early Steroidogenic Lesion in Murine Leydig Tumor Cells," Endocrinology 116, 2583- 2591. 104. D.P. Giedroc, S.K. Sinha, K. Brew, and D. Puett (1985) "Differential Trace Labeling of Calmodulin. Investigation of Binding Sites and Conformational States by Individual Lysine Reactivities," J. Biol. Chem. 260, 13406-13413. 105. S.J. Zimniski, M.E. Brandt, M.H. Melner, D.F. Covey, and D. Puett (1985) "Inhibition of Leydig Tumor Cell Steroidogenesis by 10-Propargylestr-4-ene-3,17-dione, an Irreversible Aromatase Inhibitor," Cancer Res. 45, 4883-4889. 106. D.P. Giedroc and D. Puett (1985) "Binding of a Synthetic β-Endorphin Peptide to Calmodulin," Mol. Pharmacol. 28, 588-593. 107. S.L. Young, A.E. Jackson, D. Puett, and M.H. Melner (1985) "Detection of Chloramphenicol Acetyl Transferase Activity in Transfected Cells: A Rapid and Sensitive HPLC-based Method," DNA 4, 469-475. 108. M.H. Melner, W. Voigt, and D. Puett (1986) "Cross-reactivity of a Monoclonal Antibody Directed Against an Estrogen-induced Protein in MCF-7 Human Breast Cancer Cells with Murine Leydig Cell Tumor Proteins," Cancer Res. 46, 328-331. 109. L.J. Dangott, D. Puett, and M.H. Melner (1986) "Characterization of Protein Tyrosine Kinase Activity in Murine Leydig Tumor Cells," Biochim. Biophys. Acta 886, 187-194. 110. A.E. Jackson and D. Puett (1986) "Binding of Trifluoperazine and Fluorene-containing Compounds to Calmodulin and Adducts," Biochem. Pharmacol. 35, 4395-4400. 111. S. Birken, M.A.G. Kolks, S. Amr, B. Nisula, and D. Puett (1986) "Tryptic Digestion of the Alpha Subunit of Human Choriogonadotropin." J. Biol. Chem. 261, 10719-10727. 112. A.E. Jackson, K.L. Carraway, III, D. Puett, and K. Brew (1986) "Effects of the Binding of Myosin Light Chain Kinase on the Reactivities of Calmodulin Lysines," J. Biol. Chem. 261, 12226-12232. 113. M.H. Melner, S.L. Young, F.S. Czerwiec, D. Lyn, D. Puett, D.L. Roberts, and R.D. Koos (1986) "The Regulation of Granulosa Cell Proopiomelanocortin mRNA by Androgens and Gonadotropins," Endocrinology 119, 2082-2088. 114. D.P. Giedroc, D. Puett, S.K. Sinha, and K. Brew (1987) "Calcium Effects on Calmodulin Lysine Reactivities," Arch. Biochem. Biophys. 252, 136-144. 115. J. Nieves, L. Kim, D. Puett, L. Echegoyen, J. Benabe, and M. Martinez-Maldonado (1987) "Electron Spin Resonance of Calmodulin-Vanadyl Complexes," Biochemistry 26, 4523-4527. 116. K.L. Carraway, III, Y. Liu, D. Puett, K.L. Carraway, and C.A. Carothers-Carraway (1987) "Phenothiazine Binding by a Homolog of Calpactin, the pp60 Tyrosine Kinase Substrate," FASEB J. 1, 46-50. 117. S. Birken, M.A.G. Kolks, S. Amr, B. Nisula, and D. Puett (1987) "Structural and Functional Studies of the Tryptic Core of the Human Chorionic Gonadotropin β-Subunit," Endocrinology 121, 657-666. 118. R.H. Ahmed, J. Nieves, L. Kim, L. Echegoyen, and D. Puett (1987) "Vanadyl Binding to a Testicular S-100-like Protein and to Calmodulin: Electron Paramagnetic Resonance Spectra of VO2+-Protein Complexes," J. Prot. Chem. 6, 431-439. 119. A.E. Jackson, K.L. Carraway III, M.E. Payne, A.R. Means, D. Puett, and K. Brew (1987) "Association of Calmodulin and Smooth Muscle Myosin Light Chain Kinase: Application of a Label Selection Technique with Trace Acetylated Calmodulin," Proteins: Structure, Function, and Genetics 2, 202-209. 120. A.E. Jackson, T.M. Harris, and D. Puett (1987) "Characterization of Specific Fluorenylmethyloxycarbonyl-containing Calmodulin Adducts by Spectroscopy and Phosphodiesterase Stimulation," J. Prot. Chem. 6, 497-515. 121. E.S. Lovegren, N. Ling, and D. Puett (1988) "Interaction of α-N-Acetyl-β-Endorphin and Calmodulin," J. Prot. Chem. 7, 35-47. 122. M.E. Brandt, D. Puett, D.F. Covey, and S.J. Zimniski (1988) "Characterization of Pregnant Mare's Serum Gonadotropin-Stimulated Rat Ovarian Aromatase and its Inhibition by 10-Propargylestr-4-ene-3,17-dione," J. Steroid Biochem. 31, 317-324. 123. K. Ataya, A. Weintraub, J. Dunbar, S. Zimniski, D. Puett, and W. LeMaire (1988) "Effects of LHRH Agonists on Body Weight in Rats," Drug Devel. Res. 13, 45-56. 124. G. Sanyal, L.M. Richard, K.L. Carraway III, and D. Puett (1988) "Binding of Amphiphilic Peptides to a Carboxy-terminal Tryptic Fragment of Calmodulin," Biochemistry 27, 6229-6236. 125. Q. Wei, A.E. Jackson, S. Pervaiz, K.L. Carraway III, E.Y.C. Lee, D. Puett, and K. Brew (1988) "Effects of Interaction with Calcineurin on the Reactivities of Calmodulin Lysines," J. Biol. Chem. 263, 19541-19544. 126. F.S. Czerwiec, M.H. Melner, and D. Puett (1989) "Transiently Elevated Levels of c-fos and c-myc Oncogene Messenger Ribonucleic Acids in Cultured Murine Leydig Tumor Cells after Addition of Human Chorionic Gonadotropin," Mol. Endocrinology 3, 105-109. 127. S. Bane Hastie, R.C. Williams, Jr., D. Puett, and T.L. Macdonald (1989) "The Binding of Isocolchicine to Tubulin. Mechanisms of Ligand Association with Tubulin," J. Biol. Chem. 264, 6682-6688. 128. K. Nomura, D.W. Puett, D. Puett, K. Shizume, and G.W. Liddle (1989) "Extragonadal Effects of Luteinizing Hormone in Mice," Acta Endocrinol. 121, 587-594. 129. H.G. Zot and D. Puett (1989) "An Enzymatically Active Complex of Calmodulin and Rabbit Skeletal Muscle Myosin Light Chain Kinase," J. Biol. Chem. 264, 15552-15555. 130. S. El-Deiry, D. Kaetzel, G. Kennedy, J. Nilson, and D. Puett (1989) "Site-directed Mutagenesis of the Human Chorionic Gonadotropin β Subunit: Bioactivity of a Heterologous Hormone, Bovine α-Human des-(122-145)β," Mol. Endocrinology 3, 1523-1528. 131. D. Puett and S. Birken (1989) "Helix Formation in Reduced, S-Carboxymethylated Human Choriogonadotropin β Subunit and Tryptic Peptides," J. Prot. Chem. 8, 779-794. 132. M.E. Brandt, D. Puett, and S.J. Zimniski (1990) "Divergence between Ovarian Aromatase Activity, Estrogen, and Androgen Levels in the Cycling Rat," Endocrinology 126, 72-79. 133. H.G. Zot, R. Aden, S. Samy, and D. Puett (1990) "Fluorescent Adducts of Wheat Calmodulin Implicate the Amino Terminal Region in the Activation of Skeletal Muscle Myosin Light Chain Kinase," J. Biol. Chem. 265, 14796-14801. 134. E.S. Lovegren, S.J. Zimniski, and D. Puett (1991) "Ovarian Contents of Immunoreactive β-Endorphin and α-N-Acetylated Opioid Peptides in Rats," J. Reprod. Fert. 91, 91-100. 135. S. El-Maasarany, M.E. Brandt, M.H. Majercik, S.J. Zimniski, and D. Puett (1991) "Immunocytochemical Localization and Enzymatic Distribution of Rat Ovarian Aromatase," Biol. Reprod. 44, 550-560. 136. M.H. Majercik and D. Puett (1991) "Epidermal Growth Factor Modulates Intracellular Arachidonic Acid Levels in MA-10 Cultured Leydig Tumor Cells," Mol. Cell. Endocrinology 75, 247-256. 137. S. El-Deiry, T.-M. Chen, and D. Puett (1991) "Comparison of Steroidogenic Potencies of Homologous and Heterologous Gonadotropins in Rat and Mouse Leydig Cells," Mol. Cell. Endocrinology 76, 105-113. 138. F. Chen and D. Puett (1991) "Delineation via Site-Directed Mutagenesis of the Carboxy-terminal Region of Human Choriogonadotropin β Required for Subunit Assembly and Biological Activity," J. Biol. Chem. 266, 6904-6908. 139. S.J. Zimniski, M.E. Brandt, M.H. Melner, A.M.H. Brodie, and D. Puett (1991) "Effect of 4-Hydroxyandrostenedione on Murine Leydig Tumor Cell Steroidogenesis," Cancer Res. 51, 3663-3668. 140. F. Chen, Y. Wang, and D. Puett (1991) "Role of the Invariant Asp 99 of Human Choriogonadotropin β in Receptor Binding and Biological Activity," J. Biol. Chem. 266, 19357-19361. 141. F. Chen and D. Puett (1991) "Contribution of Arginines-43 and -94 of Choriogonadotropin β to Receptor Binding and Activation as Determined by Oligonucleotide-based Mutagenesis," Biochemistry 30, 10171-10175. 142. F. Chen and D. Puett (1992) "A Single Amino Acid Residue Replacement in the β Subunit of Human Chorionic Gonadotrophin Results in the Loss of Biological Activity," J. Mol. Endocrinol. 8, 87-89. 143. F. Chen, Y. Wang, and D. Puett (1992) "The Carboxy-Terminal Region of the Glycoprotein Hormone α-Subunit: Contributions to Receptor Binding and Signaling in Human Chorionic Gonadotropin," Mol. Endocrinology 6, 914-919 (addendum: 7, 1330, 1993). 144. J. Huang, M. Ujihara, H. Xia, F. Chen, H. Yoshida, and D. Puett (1993) "Mutagenesis of the 'Determinant Loop' Region of Human Choriogonadotropin β," Mol. Cell. Endocrinology 90, 211-218. 145. A. Ergul, M.K. Glassberg, M.H. Majercik, and D. Puett (1993) "Endothelin-1 Promotes Steroidogenesis and Stimulates Protooncogene Expression in Transformed Murine Leydig Cells," Endocrinology 132, 598-603. 146. H. Xia, L.M. Fernandez, and D. Puett (1993) "Replacement of the Invariant Tyrosine in the CAGY Region of the Human Chorionic Gonadotropin β Subunit," Mol. Cell. Endocrinology 92, R1-R5. 147. H. Xia, J. Huang, T.-M. Chen, and D. Puett (1993) "Lysine Residues 2 and 104 of the Human Chorionic Gonadotrophin β Subunit Influence Receptor Binding," J. Mol. Endocrinology 10, 337-343. 148. J. Huang, F. Chen, and D. Puett (1993) "Amino/Carboxyl-terminal Deletion Mutants of Human Choriogonadotropin β," J. Biol. Chem. 268, 9311-9315. 149. S.J. Zimniski, M.E. Brandt, D.F. Covey, and D. Puett (1993) "Inhibition of Growth and Appearance of Estrogen Dependent Rat Mammary Tumors by 10-Propargylestr-4-ene-3,17-dione, an Aromatase Inhibitor," Breast Cancer Res. Treatment 26, 15-21. 150. S.P. Yadav, K. Brew, M.H. Majercik, and D. Puett (1994) "A Label Selection Approach to Assess the Role of Individual Amino Groups in Human Choriogonadotropin-Receptor Binding", J. Biol. Chem. 269, 3991-3998. 151. S.A. Stein, E.L. Oates, C.R. Hall, R.M. Grumbles, L.M. Fernandez, N.A. Taylor, D. Puett, and S.K. Jin (1994) "Identification of a Point Mutation in the Thyrotropin Receptor of the hyt/hyt Hypothyroid Mouse," Mol. Endocrinology 8, 129-138. 152. M.K. Glassberg, A. Ergul, A. Wanner, and D. Puett (1994) "Endothelin-1 Promotes Mitogenesis in Airway Smooth Muscle Cells," Amer. J. Resp. Cell Mol. Biol. 10, 316- 321. 153. H. Xia, F. Chen, and D. Puett (1994) "A Region in the Human Glycoprotein Hormone α Subunit Important in Holoprotein Formation and Receptor Binding," Endocrinology 134, 1768-1770. 154. M. Abae, M. Glassberg, M.H. Majercik, H. Yoshida, R. Vestal, and D. Puett (1994) "Immunoreactive Endothelin-1 Concentrations in Follicular Fluid of Women With and Without Endometriosis Undergoing in vitro Fertilization-Embryo Transfer," Fertility Sterility 61, 1083-1087. 155. H. Xia and D. Puett (1994) "Identification of Conserved Amino Acid Residues in the β Subunit of Human Chorionic Gonadotropin Important in Holoprotein Formation," J. Biol. Chem. 269, 17944-17953. 156. A. Ergul, M.K. Glassberg, M.E. Freeman, and D. Puett, (1994) "A Transformed Murine Leydig Cell Line Expresses the ETA Receptor Subtype", Mol. Cell. Biochem. 136, 91-96. 157. J. Huang and D. Puett (1994) "On the Role of the Invariant Glutamine at Position 54 in the Human Choriogonadotropin β Subunit", Mol. Cell. Biochem. 136, 183-186. 158. S.P. Yadav, K. Brew, and D. Puett (1994) "Holoprotein Formation of Human Chorionic Gonadotropin: Differential Trace Labeling with Acetic Anhydride," Mol. Endocrinology 8, 1547-1558. 159. A. Ergul, M.K. Glassberg, A. Wanner, and D. Puett (1995) "Characterization of Endothelin Receptor Subtypes on Airway Smooth Muscle Cells," Exp. Lung Res. 21, 453- 468. 160. D. Parra-Diaz, L. Echegoyen, H.G. Zot, and D. Puett (1995) "Vanadium (IV) Inhibits Calmodulin-Stimulated Skeletal Muscle Myosin Light Chain Kinase Activity," BioFactors 6, 1-4. 161. A. Ergul and D. Puett (1995) "Trp-21 is Important in the Processing and Secretion of Big Endothelin-1," Mol. Cell. Endocrinology 110, 89-94. 162. D.R. Maurer, J. Wimalasena, K. Indrapichate, D. Puett, and M.H. Majercik (1995) Cytoskeletal Regulation of hCG-Induced Receptor Clustering in Transformed Leydig Cells," Cell. Physiol. Biochem. 5, 361-370. 163. M.T. Kowalska, Y. Itzhak, and D. Puett (1995) "Presence of Aromatase Inhibitors in Cycads," J. Ethnopharmacol. 47, 113-116. 164. A. Ergul, R.L. Tackett, and D. Puett (1995) “Identification of Receptor Binding and Activation Sites in Endothelin-1 by Use of Site-Directed Mutagenesis,” Circulation Res. 77, 1087-1094. 165. P. Narayan, C. Wu, and D. Puett (1995) “Functional Expression of Yoked Human Chorionic Gonadotropin in Baculovirus-Infected Insect Cells,” Mol. Endocrinology 9, 1720-1726. 166. J. Huang and D. Puett (1995) "Identification of Two Amino Acid Residues on the Extracellular Domain of the Lutropin/Choriogonadotropin Receptor Important in Signaling," J. Biol. Chem. 270, 30023-30028. 167. D. Parra-Diaz, Q. Wei, E.Y.C. Lee, L. Echegoyen, and D. Puett (1995) "Binding of Vanadium (IV) to the Phosphatase Calcineurin," FEBS Letters 376, 58-60. 168. L.M. Fernandez and D. Puett (1996) “Lys583 in the Third Extracellular Loop of the Lutropin/Choriogonadotropin Receptor is Critical for Signaling,” J. Biol. Chem. 271, 925-930 (correction: p. 13926). 169. P. Narayan, J. Gray, and D. Puett (1996) “Expression of Functional Lutropin/Choriogonadotropin Receptor in the Baculovirus System,” Mol. Cell. Endocrinology 117, 95-100. 170. K. Angelova, G.B. Fralish, D. Puett, and P. Narayan (1996) “Identification of Conventional and Novel Endothelin Receptors in Sheep Choroid Plexus Cells”, Mol. Cell. Biochem. 159, 65-72. 171. L.M. Fernandez and D. Puett (1996) “Identification of Amino Acid Residues in Transmembrane Helices VI and VII of the Lutropin/Choriogonadotropin Receptor Involved in Signaling,” Biochemistry 35, 3986-3993 . 172. M. Grossmann, M.W. Szkudlinski, J.A. Dias, H. Xia, R. Wong, D. Puett, and B.D. Weintraub (1996) “Site-Directed Mutagenesis of Amino Acids 33-44 of the Common α-Subunit Reveals Different Structural Requirements for Heterodimer Expression among the Glycoprotein Hormones and Suggests that Cyclic Adenosine 3',5'-Monophosphate Production and Growth Promotion are Dissociable Functions of Human Thyrotropin,” Mol. Endocrinology 10, 769-779. 173. N. Bhowmick, J. Huang, D. Puett, N.W. Isaacs, and A.J. Lapthorn (1996) “Determination of Residues Important in Hormone Binding to the Extracellular Domain of the Luteinizing Hormone/Chorionic Gonadotropin Receptor by Site-Directed Mutagenesis and Modeling,” Mol. Endocrinology 10, 1147-1159. 174. H. Bermek, K.-C. Peng, K. Angelova, A. Ergul, and D. Puett (1996) “Endothelin Degradation by Vascular Smooth Muscle Cells,” Regulatory Peptides 66, 155-162. 175. K.-C. Peng, G.R. Bousfield, D. Puett, and D.N. Ward (1996) “Circular Dichroic Spectroscopy of Arg46-Nicked Ovine Lutropin α and Derived Fragments,” J. Prot. Chem. 15, 547-552. 176. S. Ergul, D.C. Parish, D. Puett, and A. Ergul (1996) “Racial Differences in Plasma Endothelin-1 Concentrations in Individuals with Essential Hypertension,” Hypertension 28, 652-655 (correction: 29, 912, 1997). 177. C. Wu, P. Narayan, and D. Puett (1996) “Protein Engineering of a Novel Constitutively Active Hormone-Receptor Complex”, J. Biol. Chem. 271, 31638-31642. 178. L. Shen, H. Xia, N. Bhowmick, P. Narayan, and D. Puett (1996) “Mutations of Arg68 of the Human Chorionic Gonadotrophin β Subunit Lead to Reduced Secretion,” J. Mol. Endocrinology 17, 257-262. 179. K. Angelova, A. Ergul, P. Narayan, and D. Puett (1996) “Endothelin Binding to NG108-15 Cells: Evidence for Conventional ETA and ETB Receptor Subtypes and Super-High Affinity Binding Components,” Cell. Mol. Biol. 42, 1243-1257. 180. N. Zhang, K.-C. Peng, L. Chen, D. Puett, and M. Pierce (1997) “Circular Dichroic Spectroscopy of N-Acetylglucosaminyltransferase V and Its Substrate Interactions,” J. Biol. Chem. 272, 4225-4229. 181. K.-C. Peng, D. Puett, and J.M. Brewer (1997) “Homodimer Formation by the Individual Subunits of Bovine Lutropin as Determined by Sedimentation Equilibrium,” J. Mol. Endocrinology 18, 259-265. 182. L.M. Fernandez and D. Puett (1997) “Evidence for an Important Functional Role of Intracellular Loop II of the Lutropin Receptor,” Mol. Cell. Endocrinology 128, 161-169. 183. K. Angelova, A. Ergul, K.-C. Peng, and D. Puett (1997) “Metabolism of Endothelin-1 by Neuroblastoma-Glioma Hybrid (NG108-15) Cells”, Neuroscience Letters 224, 1-4. 184. K. Angelova, D. Puett, and P. Narayan (1997) “Identification of Endothelin Receptor Subtypes in Sheep Choroid Plexus,” Endocrine 7, 287-293. 185. A. Ergul, K. Shoemaker, D. Puett, and R.L. Tackett (1998) “Gender Differences in the Expression of Endothelin Receptors in Human Saphenous Veins in vitro,” J. Pharmacol. Exp. Ther. 285, 511-517. 186. N. Bhowmick, P. Narayan, and D. Puett (1998) “Surface Retention of an Inactivating Lutropin Receptor Mutant in Exoloop 3,” Mol. Cell. Biochem. 187, 221-227. 187. N. Bhowmick, P. Narayan, and D. Puett (1998) “The Endothelin Subtype A Receptor Undergoes Agonist and Antagonist-Mediated Internalization in the Absence of Signaling”, Endocrinology 139, 3185-3192. 188. S. Ergul, A. Ergul, J.A. Hudson, D. Puett, B.M. Wieman, M.D. Durham, and D.C. Parish (1998) “The Effect of Regulation of High Blood Pressure on Plasma Endothelin-1 Levels in African-American Hypertensives,” Amer. J. Hypertension 11, 1381-1385. 189. A. Ergul, R.L. Tackett, and D. Puett (1999) “Distribution of Endothelin Receptors in Saphenous Veins of African Americans: Implications of Racial Differences,” J. Cardiovasc. Pharmacol. 34, 327-332. 190. C.A. Alvarez, P. Narayan, J. Huang, and D. Puett (1999) “Characterization of a Region of the Lutropin Receptor Extracellular Domain Near Transmembrane Helix 1 that is Important in Ligand-Mediated Signaling”, Endocrinology 140, 1775-1782. 191. N. Bhowmick, P. Narayan, and D. Puett (1999) “Identification of Ionizable Amino Acid Residues on the Extracellular Domain of the Lutropin Receptor Involved in Ligand Binding,” Endocrinology 140, 4558-4563. 192. P. Narayan, J. Gray, and D. Puett (2000) “A Biologically Active Single Chain Human Chorionic Gonadotropin Analog with Altered Receptor Binding Properties,” Endocrinology 141, 67-71. 193. P. Narayan, C. Wu, and D. Puett (2000) “Genetic Engineering of Single Chain Gonadotropins and Hormone-Receptor Fusion Proteins,” Methods 21, 59-66. 194. K. Angelova, P. Narayan, J.P. Simon, and D. Puett (2000) “Functional Role of Transmembrane Helix 7 in the Activation of the Heptahelical Lutropin Receptor,” Mol. Endocrinology 14, 459-471. M. Zhang, P. Tong, V. Fremont, J. Chen, P. Narayan, D. Puett, B.D. Weintraub, and M.W. Szkudlinski (2000) “The Extracellular Domain Suppresses Constitutive Activity of the Transmembrane Domain of the Human TSH Receptor: Implications for Hormone-Receptor Interaction and Antagonist Design,” Endocrinology 141, 3514-3517. G.B. Fralish, P. Narayan, and D. Puett (2001) “High-level Expression of a Functional Single Chain Human Chorionic Gonadotropin-Luteinizing Hormone Receptor Ectodomain Complex in Insect Cells,” Endocrinology 142, 1517-1524. J.P. Simon, K. Angelova, and D. Puett (2002) “Molecular Modeling of Transmembrane Helices 6 and 7 of the Heptahelical Lutropin Receptor,” Protein Peptide Letters 9, 153-158. K. Angelova, F. Fanelli, and D. Puett (2002) “A Model for Constitutive Lutropin Receptor Activation Based on Molecular Simulation and Engineered Mutations in Transmembrane Helices 6 and 7,” J. Biol. Chem. 277, 32202-32213. P. Narayan, J. Gray, and D. Puett (2002) “Yoked Complexes of Human Choriogonadotropin and the Lutropin Receptor: Evidence that Monomeric Individual Subunits are Inactive,” Mol. Endocrinology 16, 2733-2745. 200. K. Angelova and D. Puett (2002) “Differential Responses of an Invariant Region in the Ectodomain of the Three Glycoprotein Hormone Receptors to Mutagenesis and Assay Conditions,” Endocrine 19, 147-154. 201. R.L. Schubert, P. Narayan, and D. Puett (2003) “Specificity of Cognate Ligand-Receptor Interactions: Fusion Proteins of hCG and the Heptahelical Receptors for Human Luteinzing Hormone, Thyroid-Stimulating Hormone, and Follicle-Stimulating Hormone,” Endocrinology 144, 129-137. 202. G.B. Fralish, P. Narayan, and D. Puett (2003) “Consequences of Single-Chain Translation on the Structures of Two Chorionic Gonadotropin Yoked Analogs in α-β and β-α Configurations,” Mol. Endocrinology 17, 757-767. 203. G.B. Fralish, B. Dattilo, and D. Puett (2003) “Structural Analysis of Yoked Chorionic Gonadotropin-Luteinizing Hormone Receptor Ectodomain Complexes by Circular Dichroic Spectroscopy”, Mol. Endocrinology 17, 1192-1202. 204. K. Angelova, P. Narayan, and D. Puett (2003) “Binding and Signaling of the Luteinizing Hormone Receptor: Influence of Buffer Composition on Wild Type and Mutant Receptors,” Mol. Cell. Endocrinol. 204, 1-9. 205. R.L. Schubert and D. Puett (2003) “Single-Chain Human Chorionic Gonadotropin Analogs Containing the Determinant Loop of the b-Subunit Linked to the a-Subunit,” J. Mol. Endocrinol. 31, 157-168. 206. T.P. Meehan, D. Puett, and P. Narayan (2004) “Tightly Regulated and Inducible Expression of a Yoked Hormone-Receptor Complex in HEK 293 Cells”, J. Mol. Endocrinol. 32, 247-255. 207. K. Angelova, V. Fremont, R. Jain, M. Zhang, D. Puett, P. Narayan, and M. Szkudlinski (2004) “Human a-Subunit Analogs Act as Partial Agonists to the Thyroid-Stimulating Hormone Receptor: Differential Effects of Free and Yoked Subunits,” Endocrine 24, 25-31. 208. L.S. Kelly, M. Kozak, T. Walker, M. Pierce, and D. Puett (2005) “Lectin Immunoassays using Antibody Fragments to Human Chorionic Gonadotropin Secreted by Choriocarcinoma Cells, “Anal. Biochem. 338, 253-262. T.P. Meehan, B.G. Harmon, M.E. Overcast, K.K. Yu, S.A. Camper, D. Puett, and P. Narayan (2005) “Gonadal Defects and Hormonal Alterations in Transgenic Mice Expressing a Single Chain Human Chorionic Gonadotropin - Lutropin Receptor Complex,” J. Mol. Endocrinol. 34, 489-503. S. Kelly, S. Birken, and D. Puett (2007) “Determination of Hyperglycosylated Human Chorionic Gonadotropin Produced by Malignant Gestational Trophoblastic Neoplasias and Male Germ Cell Tumors Using a Lectin-Based Immunoassay and Surface Plasmon Resonance,” Mol. Cell Endocrinol. 260-262, 33-39. K. Angelova, F. Fanelli, and D. Puett (2008) `` Contributions of Intracellular Loops 2 and 3 of the Lutropin Receptor in Gs Coupling,`` Mol. Endocrinol. 22, 126-138. 212. J. Cui, Q. Liu, D. Puett, and Y. Xu (2008) ``Computational Prediction of Human Proteins that can be Secreted into the Bloodstream,`` Bioinformatics 24, 2370-2375. 213. S.W. Warrenfeltz, S.A. Lott, T.M. Palmer, J.A. Gray, and D. Puett (2008) ``Luteinizing Hormone-Induced Up-Regulation of ErbB-2 is Insufficient Stimulant of Growth and Invasion in Ovarian Cancer Cells,`` Mol. Cancer Res. 6, 1775-1785. 214. K. Angelova, H. de Jonge, J.C.M. Granneman, D. Puett, and J. Bogerd (2010) ``Functional Differences of Invariant and Highly Conserved Residues in the Extracellular Domain of the Glycoprotein Hormone Receptors,`` J. Biol. Chem 285, 34813-34827. 215. K. Xu, J. Cui, V. Olman, Q. Yang, D. Puett, and Y. Xu (2010) ``A Comparative Analysis of Gene-Expression Data of Multiple Cancer Types``, PLoS ONE 5, e13696. 216. J. Cui, Y. Chen, J. Chou, L. Sun, L. Chen, J. Suo, Z. Ni, M. Zhang, X. Kong, L. Hoffman, J. Kang, Y. Su, V. Olman, D. Johnson, D.W. Tench, J. Amster, R. Orlando, D. Puett, F. Li, and Y. Xu (2011) ``An Integrated Transcriptomic and Computational Analysis for Biomarker Identification in Gastric Cancer,`` Nuc. Acids Res. 39, 1197-1207. 217. C. Hong, J. Cui, Z. Ni, Y. Su, D. Puett, F. Li, and Y. Xu (2011) ``A Computational Method for Prediction of Excretory Proteins and Application to Identification of Gastric Cancer Markers in Urine``, PLoS ONE 6 (#2), e16875. 218. K. Angelova, A. Felline, M. Lee, M. Patel, D. Puett, and F. Fanelli (2011) ``Conserved Amino Acids Participate in the Structure Networks Deputed to Intramolecular Communication in the Lutropin Receptor,`` Cell. Mol. Life Sci. 68, 1227-1239. 219. J. Cui, F. Li, X. Fang, D. Puett, and Y. Xu (2011) ``Gene-Expression Signatures Can Distinguish Gastric Cancer Grades and Stages``, PLoS ONE 6 (#3), e17819. 220. G. DeMars, F. Fanelli, and D. Puett (2011) ``The Extreme C-Terminal Region of Gαs Differentially Couples to the Human Luteinizing Hormone and β2-Adrenergic Receptors.`` Mol. Endocrinol. 25, 1416-1430. 221. J. Cui, B.M. Miner, J.B. Eldredge, S.W. Warrenfeltz, Y. Xu, and D. Puett (2011) ``Gene Expression Profiling of Ovarian Cancer Cells: Alterations Due to Luteinizing Hormone Receptor Expression and Activation.`` BMC Cancer 11, 280. 222. J. Cui, J.B. Eldredge, Y. Xu, and D. Puett (2011) ``MicroRNA Expression and Regulation in Human Ovarian Cancer Cells by Luteinizing Hormone.`` PLoS ONE 6 (#7), e21730. 223. M.L. Shirley, A. Venable, R.R. Rao, N.L. Boyd, S.L. Stice, D. Puett, and P. Narayan (2012) ``Bone Morphogenetic Protein-4 Affects both Trophoblast and Non-Trophoblast Linage- Associated Gene Expression in Human Embryonic Stem Cells.`` Stem Cell Discovery 2, 163-175. 224. J.W. Layher, Jr., J.S. Poling, M. Ishihara, P. Azadi, G. Alvarez-Manilla, and D. Puett (2013) ``A Possible Effect of Concentrated Oolong Tea Causing Transient Ischemic Attack-Like Symptoms.`` Brit. J. Med. Med. Res. 3, 2157-2172. Case Report. 225. P.B. Bowman and D. Puett (2014) ``Electron Paramagnetic Resonance of Nitroxide-Labeled Calmodulin.`` (Pending). JURIED PUBLICATION IN MEDICAL EDUCATION D. Puett and J.L. Braunstein (1991) "The Endocrine Module: An Integrated Course for First-year Medical Students Combining Lecture-based and Problem-based Curricula," Teaching and Learning in Medicine 3, 159-165. BOOK CHAPTERS, REVIEWS, AND SYMPOSIUM PROCEEDINGS 1. D. Puett, M. Ascoli, and L.A. Holladay (1974) "Conformation and Metabolic Aspects of Gonadotropins," in Hormone Binding and Target Cell Activation in the Testis (M.L. Dufau and A.R. Means, eds.), Plenum Press, New York, pp. 109-124 (1974). Invited Talk and Paper. 2. L.A. Holladay and D. Puett (1975) "Physicochemical Characteristics and a Proposed Conformation of Somatostatin," in Peptides: Chemistry, Structure and Biology (R. Walter and J. Meienhofer, eds.), Ann Arbor Science Publ., Inc., Ann Arbor, pp. 175-179. 3. P.B. Hewgley and D. Puett (1980) "Spin Labeled Calmodulin: A New Probe for Studying Ca2+ and Macromolecular Interactions," Ann. New York Acad. Sci. 356, 20-32. Invited Talk and Paper. 4. T.W. Strickland, T.D. Noland, and D. Puett (1980) "Gonadotropin-Testicular Receptor Interactions and Subunit-Subunit Interactions," in Functional Correlates of Hormone Receptors in Reproduction (V.B. Mahesh, T.G. Muldoon, B.B. Saxena, and W.A. Sadler, eds.), Elsevier/North-Holland Biomed. Press, New York, pp. 463-478. Invited Talk and Paper. 5. M.H. Melner and D. Puett (1984) "Applications of Molecular Biology to Testicular Function and Regulation," in Human Fertility, Health and Food. Impact of Molecular Biology and Biotechnology. (D. Puett, ed.), United Nations Fund for Population Activities, pp. 129-140. Invited Paper. 6. M.H. Melner, D. Puett, D.L. Garbers, and L. Dangott (1984) "Testicular Tyrosine Specific Protein Kinase Activity: High Levels in Purified Leydig Cells," Ann. New York Acad, Sci. 438, 632-635. 7. F.S. Czerwiec, M.H. Melner, and D. Puett (1985) "Oncogenes: A Rediscovery of Endocrinology," in Syllabus of the Thirty-seventh Annual Postgraduate Assembly of the Endocrine Society (Miami Beach, FL), pp. 218-234. Invited Talk and Paper. 8. D. Puett (1986) "Human Choriogonadotropin," BioEssays 4, 70-75. Invited Paper. 9. D. Puett, M.E. Brandt, D.F. Covey, and S.J. Zimniski (1986) "Characterization of a Potent Inhibitor of Aromatase: Inhibition of the Rat Ovarian Enzyme and Regression of Estrogen- dependent Mammary Tumors by 10-Propargylestr-4-ene-3,17-dione,`` in Endocrinology and Malignancy. (E.E. Baulieu, S. Iacobelli, and W.L. McGuire, eds.), Parthenon Publishing, Lancester, pp. 278-289. Invited Talk and Paper. 10. M.H. Melner, S.L. Young, F. Czerwiec, D. Lyn, A. Jackson, D. Puett and R. D. Koos (1986) "Tissue-specific Hormonal Regulation of Proopiomelanocortin Gene Expression," in Advances in Gene Technology: Molecular Biology of the Endocrine System, ICSU Short Reports 4, Proc. 18th Miami Winter Symposium. (D. Puett, F. Ahmad, S. Black, D.M. Lopez, M.H. Melner, W.A. Scott, and W.J. Whelan, eds.) Cambridge University Press, Cambridge, pp. 10-13. Invited Paper. 11. S.J. Zimniski, M.E. Brandt, D.F. Covey, and D. Puett (1987) "Inhibition of Aromatase Activity and of Endocrine-responsive Tumor Growth by 10-Propargylestr-4-ene-3,17-dione and its 17-Proprionate Derivative," Steroids 50, 135-146. Invited Talk and Paper. 12. M.E. Brandt, D. Puett, R. Garola, K. Fendl, D.F. Covey, and S.J. Zimniski (1988) "Aromatase and Aromatase Inhibitors: From Enzymology to Selective Chemotherapy," in Hormones, Cell Biology and Cancer: Perspectives and Potentials. (W.D. Hankins and D. Puett, eds.), Progress in Clinical and Biological Research, Vol. 262, Alan R. Liss Publ. Co., New York, pp. 65-84. Invited Talk and Paper. 13. M.H. Majercik, F.S. Czerwiec, and D. Puett (1989) "Effects of Hormones and Intracellular Mediators on Differentiated Functions of Cultured Leydig Tumor Cells, Steroids 54, 627-645. 14. G. Sanyal, F.M. Thompson, and D. Puett (1990) "Fluorescence Spectroscopic Studies of Tyrosine Environment and Ligand Binding of Plant Calmodulin," in Time-Resolved Laser Spectroscopy in Biochemistry II, SPIE Vol. 1204 (J. Lakowicz, ed.), Plenum Publ. Co., pp. 611-627. 15. G. Sanyal, D. Puett, and F.G. Prendergast (1991) "Structural Complementarity of the Interacting Surfaces of Calmodulin and its Peptide Ligands," in Biomembranes in Health and Disease, Vol. 1 (A.M. Kidwai, R.K. Upreti, and P.K. Ray, eds.), Today & Tomorrow's Printers & Publishers, New Delhi, pp. 305-315. Invited Talk and Paper. 16. D. Puett, F. Chen, S. El-Deiry, Y. Wang, and H. Xia (1991) "Site-directed Mutagenesis of hCGβ," Advances in Reproductive Biology, Selected Lectures of the Beijing International Symposium on Reproductive Biology (Y. Cao and C. Zhu, eds.), China Society of Reproductive Biology, pp. 34-47. Invited Talk and Paper. 17. J.W. Lustbader, D.L. Yarmush, S. Birken, D. Puett, and R.E. Canfield (1993) "The Application of Chemical Studies of Human Chorionic Gonadotropin to Visualize Its Three-Dimensional Structure," Endocrine Rev. 14, 291-311. Juried Review. 18. D. Puett (1993) "Choriogonadotropin," in Human Protein Data, 2nd Installment (A. Haeberli, ed.), VCH Publ. (Updated 1997). Invited Contribution. 19. D. Puett, J. Huang, and H. Xia (1994) "Delineation of Subunit and Receptor Contact Sites by Site-Directed Mutagenesis of hCGβ," in Glycoprotein Hormones: Structure, Function and Clinical Implications (J.W. Lustbader, D. Puett, and R.W. Ruddon, eds.), Springer-Verlag, New York, pp. 122-134. Invited Talk and Paper. 20. A. Ergul and D. Puett (1995) "Mutagenesis of the Processing Site of Big Endothelin-1," in Endothelins in Endocrinology: New Advances (E. Baldi, M. Maggi, I.T. Cameron, and M.J. Dunn, eds.), Ares-Serono Symposia Publications, Rome, pp. 17-20. 21. D. Puett, N. Bhowmick, L.M. Fernandez, J. Huang, C. Wu, and P. Narayan (1996) “hCG-Receptor Binding and Transmembrane Signaling,” Mol. Cell. Endocrinology 125, 55-64. Invited Talk and Paper. 22. D. Puett, C. Wu, and P. Narayan (1998) “The Tie that Binds: Design of Biologically Active Single Chain Human Chorionic Gonadotropins and a Gonadotropin-Receptor Complex Using Protein Engineering,” Biol. Reprod. 58, 1337-1342. Invited Juried Review. 23. B. Berlin, E.A. Berlin, J.C. Fernandez Ugalde, L. Garcia Barrios, D. Puett, R. Nash, and M. Gonzalez-Espinoza (1999) “The Maya ICBG: Drug Discovery, Medical Ethnobiology and Alternative Forms of Economic Development in the Highland Maya Region of Chiapas, Mexico,” Pharmaceut. Biol. 37, 127-144. 24. D. Puett (1999) “Gonadotropin Receptors,” in Encyclopedia of Reproduction (E. Knobil and J.D. Neill, eds.), Vol. 2, Academic Press, San Diego, pp. 521-528. Invited Chapter. 25. A. Ergul and D. Puett (1999) “Hormone Receptors, Overview,” in Encyclopedia of Reproduction (E. Knobil and J.D. Neill, eds.), Vol. 2, Academic Press, San Diego, pp. 654-662. Invited Chapter. . 26. D. Puett and P. Narayan (2000) “Designer Gonadotropins and Receptors: The Prospect of Recombinant Technology,” in Ovulation: Evolving Scientific and Clinical Concepts (E.Y. Adashi, ed.), Springer-Verlag, New York, pp. 295-307. Invited Chapter. 27. B. Berlin, E.A. Berlin, L. Garcia, M. González, D. Puett, R. Nash, C Montes, R.Trujillo, L.-A. Ramírez, J. Hernández, M.-Gpe. Ramírez, H. Wetzstein, T. Murray, R. Sharma, J. Affolter, and M. Heinrich (2001) “Etnobotánica Médica y Desarrollo Sustentable: El Case del ICBG-Maya en los Altos de Chiapas,” in Plantas, Cultura y Sociedad: Estudios Sobre la Relacíon entre Seres Humanos y Plantas en los Albores del Siglo Xxl (B. Rendón, S. Rebollar, J. Caballero, and M.-A. Martínez, eds.), Universida Autónoma Metropolitana-Iztapalapa, México, pp. 221-258. Invited Chapter. F. Fanelli, A.P.N. Themmen, and D. Puett (2001) “Lutropin Receptor Function: Insights from Natural, Engineered, and Computer-Simulated Mutations,” IUBMB Life 51, 1-7. Invited Review. D. Puett and P. Narayan (2002) “Fusion Proteins of Human Gonadotropins and Gonadotropin-Receptor Complexes,” Ind. J. Exp. Biol. 40, 415-423. Invited Review. F. Fanelli and D. Puett (2002) “Structural Aspects of the Luteinizing Hormone Receptor: Information from Molecular Modeling and Mutagenesis,” Endocrine 18, 285-293. P. Narayan and D. Puett (2003) “Luteinizing Hormone Receptor Signaling,” in Encyclopedia of Hormones. (H.L. Henry and A.W. Norman, eds.) Academic Press, San Diego, II, 612-616. Invited Chapter. D. Puett, Y. Li, K. Angelova, G. DeMars, T.P. Meehan, F. Fanelli, and P. Narayan (2005) “Structure-Function Relationships of the Luteinizing Hormone Receptor”, Ann. N.Y. Acad. Sci. 1061, 41-54. Invited Review. 33. D. Puett, Y. Li, G. DeMars, K. Angelova, and F. Fanelli (2007) “A Functional Transmembrane Complex: The Luteinizing Hormone Receptor with Bound Ligand and G Protein,” Mol. Cell. Endocrinol. 260-262, 126-136. Invited Review. 34. M. Ascoli and D. Puett (2009) ``The Gonadotropin Hormones and Their Receptors,`` in Yen and Jaffe`s Reproductive Endocrinology: Physiology, Pathophysiology, and Clinical Management. (Sixth Edition). J.F. Strauss, III and R. Barbieri (eds.). Elsevier, Philidelphia, 35-55. Invited Review. 35. D. Puett and K. Angelova (2009) ``Determining the Affinity of Hormone-Receptor Interaction,`` in Molecular Endocrinology: A Comprehensive Guide to Current Methodologies. O.K. Park-Sarge and Thomas E. Curry, Jr. (eds.). Methods in Molecular Biology 590, 1-20, Humana Press, Inc., Totaowa, NJ. Invited Review. 36. D. Puett, K. Angelova, M.R. da Costa, S.W. Warrenfeltz, and Francesca Fanelli (2010) ``The Luteinizing Hormone Receptor: Insights into Structure-Function Relationships and Hormone-Receptor-Mediated Changes in Gene Expression in Ovarian Cancer Cells, Mol. Cell. Endocrinol. 329, 47-55. Invited Review. 37. J. Cui, Y. Xu, and D. Puett (2012) ``Transcriptomic Analysis of Human Ovarian Cancer Cells: Changes Mediated by Luteinizing Hormone Receptor Activation,`` in Ovarian Cancer/ - Basic Science Perspective. Samir A. Farghaly (ed.). InTech Open Access Publisher, New York, NY, pp. 195-212. Invited Review. 38. J. Cui, Y. Xu, and D.Puett (2013) ``Transcriptome Profiling of Ovarian Cancer Cells,`` in Ovarian Cancer: Methods and Protocols. Series: Methods in Molecular Biology 1049, Chap.11. Anastasia Malek and Oleg Tchernitsa (eds.). Humana Press-Springer, New York, NY, pp. 119-137. Invited Review. SHORT REPORTS J.P. Robinson and D. Puett (1973) "Characterization of Tamm-Horsfall Urinary Glycoprotein," in Proceedings of the Electron Microscope Society of America (C.J. Arceneaux, ed.), Claitor's, Inc., LA, pp. 420-421. R.G. Hammonds, Jr., D. Puett, and N. Ling (1977) "Circular Dichroism of Opioid Peptides," in Peptides (M. Goodman and J. Meienhofer, eds.), John Wiley and Sons, Inc., New York, pp. 100-102. M.H. Melner and D. Puett (1984) "The Expression of a Pro-opiomelanocortin-like Gene in Murine Leydig Tumor Cells," in Advances in Gene Technology: Human Genetic Disorders, ICSU Short Reports 1, Proc. 16th Miami Winter Symposium. (W.A. Scott, F. Ahmad, S. Black, J. Schultz, and W.J. Whelan, eds.), Cambridge University Press, Cambridge, pp. 206-207. L.J. Dangott, D. Puett, and L.W. Cunningham (1984) "Changes in α2-Macroglobulin Conformation by Reaction with Trypsin and Methylamine," Ann. New York Acad. Sci. 421, 158-159. S.L. Young, A.E. Jackson, D. Puett, and M.H. Melner (1985) "A Rapid HPLC Method for Determining the Expression of CAT Genes in Transfected Cells," in Advances in Gene Technology: Molecular Biology of the Immune System, ICSU Short Reports 2 Proc. 17th Miami Winter Symposium. (J.W. Streilein, F. Ahmad, S. Black, B. Blomberg, and R.W. Voellmy, eds.), Cambridge University Press, Cambridge, pp. 351-352. E. Lovegren, S.J. Zimniski, and D. Puett (1986) "Immunoreactive β-Endorphin in the Gonadotropin-primed Immature Rat Ovary," in Advances in Gene Technology: Molecular Biology of the Endocrine System, ICSU Short Reports 4, Proc. 18th Miami Winter Symposium. (D. Puett, F. Ahmad, S. Black, D.M. Lopez, M.H. Melner, W.A. Scott, and W.J. Whelan, eds.), Cambridge University Press, Cambridge, pp. 48-49. M.E. Brandt, K.M. Wilson, S. Zimniski and D. Puett (1987) "Developmental Changes of Rat Ovarian Aromatase," in Advances in Gene Technology: The Molecular Biology of Development, ICSU Short Reports 7, Proc. 19th Miami Winter Symposium. (R.W. Voellmy, F. Ahmad, S. Black, D.R. Burgess, R. Rotundo, W.A. Scott, and W.J. Whelan, eds.), Cambridge University Press, Cambridge, p. 71. M.E. Brandt, K.M. Wilson, S.J. Zimniski, and D. Puett (1987) "Ontogeny and Stimulation of Rat Ovarian Aromatase," Steroids 50, 611-612. H.G. Zot and D. Puett (1988) "Crosslinking of Calmodulin to Troponin I and Myosin Light Chain Kinase with Carbodiimides," in Advances in Gene Technology: Protein Engineering and Production, ICSU Short Reports 8 Proc. Miami Bio/Technology Winter Symposium, p. 161. R. Batchelder and D. Puett (1989) "β-Endorphin Binding to a Neuroblastoma-Glioma Hybrid Cell Line (NG-108)," in Advances in Gene Technology: Molecular Neurobiology and Neuropharmacology, ICSU Short Reports 9, Proc. Miami Bio/Technology Winter Symposium, p. 112. R. Batchelder, R. Levy, and D. Puett (1990) "Beta-Endorphin Binding and Effects on Prostaglandin E2 Production in a Murine Macrophage Line (P388D.1)," in Advances in Gene Technology: The Molecular Biology of Immune Diseases and the Immune Response, ICSU Short Reports 10, Proc. Miami Bio/Technology Winter Symposium, p. 46. M.T. Kowalska, M.E. Brandt, and D. Puett (1990) "Inhibition of Cytochrome P-450 Aromatase Activity by Plant Extracts," Planta Medica 56, 675. M.T. Kowalska and D. Puett (1990) "Potential Biomedical Applications for Tropical Fruit Products," Tropical Fruit World 1, 126-127. T.-M. Chen and D. Puett (1991) "Hormonal Stimulation of mRNA Levels for the Oncogenes c-jun and junB in Leydig Tumor Cells," in Advances in Gene Technology, The Molecular Biology of Human Genetic Disease, Miami Short Reports 1, Proc. Miami Bio/Technology Winter Symposium, p. 75. M.H. Majercik, H. Xia, and D. Puett (1991) "Human Choriogonadotropin Stimulates the Secretion of an Extracellular Matrix-Modulating Protein from Leydig Tumor Cells," in Advances in Gene Technology: The Molecular Biology of Human Genetic Disease, Miami Short Reports 1, Proc. Miami Bio/Technology Winter Symposium, p. 80. M.T. Kowalska, Y. Itzhak, D. Cristante, and D. Puett (1992) "Aromatase Inhibitors in Edible Tropical Plants," Advances in Gene Technology: Feeding the World in the 21st Century, Miami Short Reports 2, Proc. Miami Bio/Technology Winter Symposium, p. 101. N. Bhowmick, P. Narayan, and D. Puett (1999) “An Endothelin-A/Lutropin Chimeric Receptor Exhibits Ligand-Mediated Internalization in the Absence of Signaling,” in Peptides: Frontiers of Peptide Science (J.P. Tam and P.T. P. Kaumaya, eds.), Kluwer Academic Publishers, Dordrecht (The Netherlands), pp. 584-585. F. Fanelli, A. Themmen, and D. Puett (2001) “Understanding the Activation of the Lutropin Receptor from Natural, Engineered, and Computer-Simulated Mutations,” in Proceedings of the Telethon Scientific Convention 2001, Palazzo dei Convegni Riva del Garda, pp. 67-68. ESSAY D. Puett (2005) “How I Became a Biochemist,” IUBMB Life 57, 769-771. Invited Essay. BOOKS AND JOURNALS (SPECIAL ISSUES) EDITED D. Puett, ed., Human Fertility, Health and Food. Impact of Molecular Biology and Biotechnology, (1984) United Nations Fund for Population Activities. Invited Book. D. Puett, F. Ahmad, S. Black, D.M. Lopez, M.H. Melner, W.A. Scott, and W.J. Whelan, eds., Advances in Gene Technology: Molecular Biology of the Endocrine System, ICSU Short Reports 4, Proc. 18th Miami Winter Symposium. (1986) Cambridge University Press, Cambridge. W.D. Hankins and D. Puett, eds., Hormones, Cell Biology and Cancer: Perspectives and Potential (1988) Progress in Clinical and Biological Research, Vol. 262, Alan R. Liss Publ. Co., New York. K. Brew, F. Ahmad, H. Bialy, S. Black, R.E. Fenna, D. Puett, W.A. Scott, J. Van Brunt, R.W. Voellmy, W.J. Whelan, and J.F. Woessner, eds., Advances in Gene Technology: Protein Engineering and Production, Proc. Miami Bio/Technology Winter Symposium, ICSU Short Reports 8. (1988) IRL Press, Washington, D.C. D. Puett, ed., Proceedings of the W. J. LeMaire Symposium on Reproductive Endocrinology (1989) Steroids 54, November and December issues. R.R. Howell, F. Ahmad, H. Bialy, S. Black, D.H. Johnson, H.A. Lubs, D. Puett, M.B. Rabin, W.A. Scott, J. Van Brunt, and W.J. Whelan, eds., Advances in Gene Technology: The Molecular Biology of Human Genetic Disease, Proc. Miami Bio/Technology Winter Symposium, Nucleic Acids Symposium Series, no. 23 (1991) IRL Press, Oxford. J.W. Lustbader, D. Puett, and R.W. Ruddon, eds., Glycoprotein Hormones: Structure, Function and Clinical Implications (1994) Springer-Verlag, New York. D. Puett, ed., Three issues of Mol. Cell. Endocrinol. (2007) 260-262, Transactions of the International Conference on Gonadotropins and Receptors. These issues contain 37 original and review articles. Invited Guest Editorship. PUBLISHED ABSTRACTS FOR MEETING PRESENTATIONS AND POSTERS (ABOUT 250-260) HAVE NOT BEEN RECORDED
