TitlePhotoaffinity labeling of Ras converting enzyme 1 (Rce1p) using a benzophenone-containing peptide substrate.
Publication TypeJournal Article
Year of Publication2010
AuthorsKyro K, Manandhar SP, Mullen D, Schmidt WK, Distefano MD
JournalBioorg Med Chem
Volume18
Issue15
Pagination5675-84
Date Published2010 Aug 1
ISSN1464-3391
KeywordsAmino Acid Sequence, Benzophenones, Endopeptidases, Fluorescent Dyes, Kinetics, Peptides, Protein Prenylation, Spectrometry, Mass, Electrospray Ionization, Substrate Specificity
AbstractIsoprenylation is a post-translational modification that increases protein hydrophobicity and helps target certain proteins to membranes. Ras converting enzyme 1 (Rce1p) is an endoprotease that catalyzes the removal of a three residue fragment from the C-terminus of isoprenylated proteins. To obtain structural information about this membrane protein, photoaffinity labeling agents are being prepared and employed. Here, we describe the synthesis of a benzophenone-containing peptide substrate analogue for Rce1p. Using a continuous spectrofluorometric assay, this peptide was shown to be a substrate for Rce1p. Mass spectrometry was performed to confirm the site of cleavage and structure of the processed probe. Photolysis of the biotinylated compound in the presence of membranes containing Rce1p followed by streptavidin pull-down and Western blot analysis indicated that Rce1p had been labeled by the probe. Photolysis in the presence of both the biotinylated, benzophenone-containing probe and a farnesylated peptide competitor reduced the extent of labeling, suggesting that labeling is occurring in the active site.
DOI10.1016/j.bmc.2010.06.024
Alternate JournalBioorg. Med. Chem.
PubMed ID20619662