West, Christopher M.
Professor and Head Biochemistry and Molecular Biology

Franklin School of Arts and Sciences
The University of Georgia
 
 

 
OXYGEN SENSING: In addition to its role in driving oxidative metabolism, ambient O2 levels carry information of great interest to cells of both unicellular and multicellular organisms. For example, O2 regulates gene expression and modulates ion transport across membranes, and cells can migrate toward or against O2 and metabolically adapt to changes in O2-levels. An important mechanism of O2 sensing in animals involves a cytoplasmic/nuclear prolyl 4-hydroxylase (PHD), an O2-dependent enzyme that modifies selected Pro-residues in the transcription factor subunit HIFlpha. We have discovered that PHDs also mediate O2-signaling in unicellular organisms, using the NIH model organism Dictyostelium. Dictyostelium is a social soil amoeba that, in response to nutritional deprivation, aggregates to form a multicellular slug that migrates to the soil surface to form a fruiting body (Fig. 1). An important factor that regulates conversion of the slug to a fruiting body, and the differentiation of spores in the head of the fruiting body, is sufficient O2 (Fig. 2). In the context of its environment, it is likely that the local O2 level is used by the slug to know where it is. Our work has shown that, in contrast to animals in which PHDs trigger the ubiquitination and proteasomal degradation of the HIFlpha transcription factor subunit, protist PHDs (referred to as PhyA) appear to regulate the activity of Skp1, a subunit of the SCF class of E3-ubiquitin ligases (Fig. 3) that control the stability of critical regulatory proteins such as cAMP phosphodiesterase. Current studies are probing the ways in which PhyA mediates sensing of O2 and metabolic states, and how control of Skp1 is integrated with other environmental signaling pathways that control starvation-induced developmental progression. We are also investigating a predicted lysyl hydroxylase implicated in epigenetic regulation because it associates with Skp1 and, like PhyA, this jumonji-C domain containing protein is likely subject to environmental and metabolic regulation. Dictyostelium is an excellent experimental surrogate for our parallel studies on eukaryotic pathogens of humans including the apicomplexan Toxoplasma gondii, and of plants including the oomycetes Phytophthora and Pythium. For example, in studies with Ira Blader (Buffalo), we found that phyA of Toxoplasma can substitute for the Dictyostelium gene to support O2-sensing during development (Fig. 4).

 
 
Fig. 1. Fruiting bodies. Light micrograph shows
the aerial sorus containing ~30,000 spores
supported by a 1-mm long stalk consisting
of ~7000 stalk cells.
 

 
 


.
Fig. 2. Low O2 blocks culmination.
Developing cells are stalled at the slug stage in 10% O2,
but form normal fruiting bodies at 21% O2. Thus sufficient O2
is a checkpoint for completion of development. See West et al
(2007) Development 134:3349 .

Fig. 3. Cartoon of an SCF-type E3 ubiquitin ligase.
Shown at the right is the donation of Ub-chain to a Cys of the E2-subunit,
which will transfer the Ub to the target substrate docked onto the F-box protein
receptor (via its phosphodegron), which is in turn linked to the E2 via Skp1, Cullin-1,
and Rbx1. Multiple cycles of K48 ubiquitination generate a signal that is recognized
for degradation by the 26S-proteasome. Skp1 is subject to novel regulation by
hydroxylation of Pro143, and subsequent glycosylation (colored symbols on left),
which promote the interaction of Skp1 with F-box protein substrate receptors.
Adapted from Y. Yoshida.

 


Fig. 4. Toxoplasma PhyA can replace its
Dictyostelium homolog.
At 18% O2, phyA-
KO cells remain as slugs, whereas the normal
Ax3 strain forms fruiting bodies. The phyA-KO
cells are rescued by overexpression of
Toxoplasma (Tg) PhyA, but not a catalytically
dead mutant. See Xu et al (2012) JBC 287:25098.

GLYCOREGULATION IN THE CYTOPLASM AND THE NUCLEUS: Glycosylation, best known as a major posttranslational modification of secretory, cell surface and many internal membrane proteins, is also a prevalent modification of intracellular cytoplasmic and nuclear proteins. We have discovered an O-glycosylation pathway of unprecedented sophistication for the cytoplasm, which mediates assembly of a novel linear pentasaccharide on Skp1 (Fig. 5). The pentasaccharide is assembled on the hydroxyproline generated by PhyA, and appears to have co-evolved with prolyl hydroxylation of Skp1 across unicellular eukaryotes. Our findings in Dictyostelium indicate that each sugar in the glycan has a distinguishable developmental function that modulates O2-sensing by PhyA in a hierarchical fashion. Mass spectrometric and gene tagging studies have shown that the Skp1 interactome is modified by glycosylation, which supports increased assembly of SCF complexes. The effect was partially recapitulated by in vitro studies with recombinant Skp1 glycoforms and a model F-box protein. Biophysical studies on Skp1 glycoforms including circular dichroism (with Karla Rodgers), small angle X-ray scattering (with Blaine Mooers), and 1H/15N-HSQC-NMR (with Brad Bendiak and Jim Prestegard), reveal dramatic changes in conformation. Taking a reductionist approach, studies in collaboration with Carol Taylor (LSU) show that hydroxylation and glycosylation influence the conformation of model Skp1 dipeptides (Fig. 6). Remarkably, the terminal glycosyltransferase AgtA is a Skp1-binding protein whose inhibitory effects on Skp1 appear to be moderated by glycosylation (Fig. 7).

 

 

Fig. 6. Conformational effects of Pro143 modification.
NMR studies on capped synthetic dipeptides show that
Pro hydroxylation and alphaGlcNAcylation promote, in
additive fashion, the exo-state of the pyrrolidine ring, and
the transconforma