TitleImpact of substrate glycoside linkage and elemental sulfur on bioenergetics of and hydrogen production by the hyperthermophilic archaeon Pyrococcus furiosus.
Publication TypeJournal Article
Year of Publication2007
AuthorsChou C-J, Shockley KR, Conners SB, Lewis DL, Comfort DA, Adams MWW, Kelly RM
JournalAppl Environ Microbiol
Volume73
Issue21
Pagination6842-53
Date Published2007 Nov
ISSN0099-2240
KeywordsArchaea, Chemical Phenomena, Chemistry, Gene Expression Regulation, Archaeal, Genome, Archaeal, Glycosides, Hot Temperature, Hydrogen, Oligonucleotide Array Sequence Analysis, Open Reading Frames, Pyrococcus furiosus, Sulfur
AbstractGlycoside linkage (cellobiose versus maltose) dramatically influenced bioenergetics to different extents and by different mechanisms in the hyperthermophilic archaeon Pyrococcus furiosus when it was grown in continuous culture at a dilution rate of 0.45 h(-1) at 90 degrees C. In the absence of S(0), cellobiose-grown cells generated twice as much protein and had 50%-higher specific H(2) generation rates than maltose-grown cultures. Addition of S(0) to maltose-grown cultures boosted cell protein production fourfold and shifted gas production completely from H(2) to H(2)S. In contrast, the presence of S(0) in cellobiose-grown cells caused only a 1.3-fold increase in protein production and an incomplete shift from H(2) to H(2)S production, with 2.5 times more H(2) than H(2)S formed. Transcriptional response analysis revealed that many genes and operons known to be involved in alpha- or beta-glucan uptake and processing were up-regulated in an S(0)-independent manner. Most differentially transcribed open reading frames (ORFs) responding to S(0) in cellobiose-grown cells also responded to S(0) in maltose-grown cells; these ORFs included ORFs encoding a membrane-bound oxidoreductase complex (MBX) and two hypothetical proteins (PF2025 and PF2026). However, additional genes (242 genes; 108 genes were up-regulated and 134 genes were down-regulated) were differentially transcribed when S(0) was present in the medium of maltose-grown cells, indicating that there were different cellular responses to the two sugars. These results indicate that carbohydrate characteristics (e.g., glycoside linkage) have a major impact on S(0) metabolism and hydrogen production in P. furiosus. Furthermore, such issues need to be considered in designing and implementing metabolic strategies for production of biofuel by fermentative anaerobes.
DOI10.1128/AEM.00597-07
Alternate JournalAppl. Environ. Microbiol.
PubMed ID17827328